- PDB-2vde: Crystal Structure of the Open State of TolC Outer Membrane Compon... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 2vde
タイトル
Crystal Structure of the Open State of TolC Outer Membrane Component of Mutlidrug Efflux Pumps
要素
OUTER MEMBRANE PROTEIN TOLC
キーワード
TRANSPORT PROTEIN / OUTER MEMBRANE / ALPHA HELICAL BARREL / MULTIDRUG EFFLUX PUMP / INTEGRAL MEMBRANE PROTEIN / MEMBRANE / TRANSPORT / BETA BARREL / TRANSMEMBRANE
機能・相同性
機能・相同性情報
MacAB-TolC complex / enterobactin transport / bile acid transmembrane transporter activity / xenobiotic detoxification by transmembrane export across the cell outer membrane / efflux pump complex / periplasmic side of plasma membrane / xenobiotic detoxification by transmembrane export across the plasma membrane / bile acid and bile salt transport / porin activity / efflux transmembrane transporter activity ...MacAB-TolC complex / enterobactin transport / bile acid transmembrane transporter activity / xenobiotic detoxification by transmembrane export across the cell outer membrane / efflux pump complex / periplasmic side of plasma membrane / xenobiotic detoxification by transmembrane export across the plasma membrane / bile acid and bile salt transport / porin activity / efflux transmembrane transporter activity / monoatomic ion transmembrane transport / cell outer membrane / response to organic cyclic compound / response to toxic substance / monoatomic ion channel activity / outer membrane-bounded periplasmic space / response to xenobiotic stimulus / response to antibiotic / identical protein binding / membrane 類似検索 - 分子機能
Type I secretion outer membrane protein, TolC / Outer membrane efflux proteins (OEP) / Outer membrane efflux proteins (OEP) / Outer membrane efflux protein / Outer membrane efflux protein / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
ENGINEERED RESIDUE IN CHAIN A, VAL 191 TO LEU ENGINEERED RESIDUE IN CHAIN A, TYR 384 TO PHE ...ENGINEERED RESIDUE IN CHAIN A, VAL 191 TO LEU ENGINEERED RESIDUE IN CHAIN A, TYR 384 TO PHE ENGINEERED RESIDUE IN CHAIN A, ARG 389 TO GLU ENGINEERED RESIDUE IN CHAIN B, VAL 191 TO LEU ENGINEERED RESIDUE IN CHAIN B, TYR 384 TO PHE ENGINEERED RESIDUE IN CHAIN B, ARG 389 TO GLU ENGINEERED RESIDUE IN CHAIN C, VAL 191 TO LEU ENGINEERED RESIDUE IN CHAIN C, TYR 384 TO PHE ENGINEERED RESIDUE IN CHAIN C, ARG 389 TO GLU
非ポリマーの詳細
CHLORIDE ION (CL): CHLORIDE DEDUCED FROM COORDINATION PATTERN
-
実験情報
-
実験
実験
手法: X線回折 / 使用した結晶の数: 6
-
試料調製
結晶
マシュー密度: 3.83 Å3/Da / 溶媒含有率: 67.91 % / 解説: NONE
結晶化
pH: 10.5 詳細: PROTEIN WAS CRYSTALLIZED FROM 0.1M CAPS PH10.5, 30% PEG400
解像度: 3.2→22.08 Å / Cor.coef. Fo:Fc: 0.885 / Cor.coef. Fo:Fc free: 0.839 / 交差検証法: THROUGHOUT / ESU R Free: 0.541 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. DISORDERED REGIONS WERE MODELED IN FULL WITH OCCUPANCY ZERO.
Rfactor
反射数
%反射
Selection details
Rfree
0.315
1906
5 %
RANDOM
Rwork
0.266
-
-
-
obs
0.268
36034
96.7 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK