- PDB-2v5l: Structures of the Open and Closed State of Trypanosomal Triosepho... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2v5l
Title
Structures of the Open and Closed State of Trypanosomal Triosephosphate Isomerase: as Observed in a New Crystal Form: Implications for the Reaction Mechanism
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 81 / Source method: isolated from a natural source / Formula: H2O
Sequence details
IN HOUSE NAME P4MAS_W3TB.PDB (RESIDUES 203 AND 503 ARE NOW AN ALANINE) RELATED PDB'S 1TPD AND 1TRD. ...IN HOUSE NAME P4MAS_W3TB.PDB (RESIDUES 203 AND 503 ARE NOW AN ALANINE) RELATED PDB'S 1TPD AND 1TRD. THIS IS AN OLD STRUCTURE THAT WAS ALREADY USED IN PUBLICATION NOBLE, M.E.M., ZEELEN, J.P., WIERENGA, R.K. (1993) PROTEINS, 16, 311-326. IN THAT ARTICLE IT WAS REFERRED WITH IN-HOUSE NAME P4MAS_W3TB.PDB. WE WANT TO DEPOSIT THIS STRUCTURE, BECAUSE SOME DETAILS OF IT HAVE BECOME RELEVANT FOR OUR FORTHCOMING PUBLICATION. ONLY THE COORDINATES AND THE INFORMATION ON THE ARTICLE FROM 1993 ARE AVAILABLE FOR US. THIS MEANS THAT MOST OF THE INFORMATION FOR THE HEADER FILES IS NOT AVAILABLE.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.7 Å3/Da / Density % sol: 54.87 % / Description: NONE
Crystal grow
pH: 8.8 Details: 18% PEG6000, 200 MM TRIS PH 8.8, 1MM EDTA, 1MM DTT AND 1MM NAN3
Method to determine structure: MOLECULAR REPLACEMENT Starting model: NONE Resolution: 2.4→15 Å / Cross valid method: THROUGHOUT / σ(F): 999 / Details: X-PLOR USED IN THE INITIAL STAGES OF REFINEMENT /
Rfactor
Num. reflection
Rwork
0.158
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obs
0.158
39764
Refinement step
Cycle: LAST / Resolution: 2.4→15 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
3766
0
10
81
3857
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