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- PDB-1ttj: THREE NEW CRYSTAL STRUCTURES OF POINT MUTATION VARIANTS OF MONOTI... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ttj | ||||||
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Title | THREE NEW CRYSTAL STRUCTURES OF POINT MUTATION VARIANTS OF MONOTIM: CONFORMATIONAL FLEXIBILITY OF LOOP-1,LOOP-4 AND LOOP-8 | ||||||
![]() | TRIOSEPHOSPHATE ISOMERASE | ||||||
![]() | ISOMERASE(INTRAMOLECULAR OXIDOREDUCTASE) | ||||||
Function / homology | ![]() glycosome / triose-phosphate isomerase / triose-phosphate isomerase activity / glyceraldehyde-3-phosphate biosynthetic process / glycerol catabolic process / gluconeogenesis / glycolytic process / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Radha Kishan, K.V. / Wierenga, R.K. | ||||||
![]() | ![]() Title: Three new crystal structures of point mutation variants of monoTIM: conformational flexibility of loop-1, loop-4 and loop-8. Authors: Borchert, T.V. / Kishan, K.V. / Zeelen, J.P. / Schliebs, W. / Thanki, N. / Abagyan, R. / Jaenicke, R. / Wierenga, R.K. #1: ![]() Title: The 2.4 Angstrom Crystal Structure of the Complex of Monoss (A Monomeric Variant of Trypanosomal Triosephosphate Isomerase) and a Transition State Analogue, Phosphoglycolohydroxamate Authors: Radha Kishan, K.V. / Ph Zeelen, J. / Schliebs, W. / Wierenga, R.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57.2 KB | Display | ![]() |
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PDB format | ![]() | 41 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 387.9 KB | Display | ![]() |
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Full document | ![]() | 396.4 KB | Display | |
Data in XML | ![]() | 7.2 KB | Display | |
Data in CIF | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26020.688 Da / Num. of mol.: 1 Mutation: VARIANT OF MONOTIM WITH PHE 45 REPLACED BY SER AND VAL 46 REPLACED BY SER (F45S, V46S) AND 73 - 79 DELETED Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-PGH / |
#3: Water | ChemComp-HOH / |
Sequence details | THERE IS ONE BREAK IN THE SEQUENCE AT 73. RESIDUE NUMBERS 73 - 79 ARE MISSING DUE TO DELETION OF A ...THERE IS ONE BREAK IN THE SEQUENCE AT 73. RESIDUE NUMBERS 73 - 79 ARE MISSING DUE TO DELETION OF A LOOP FROM THE PARENT MODEL AND CONNECTING |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.92 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Resolution: 2.4→32 Å / Num. obs: 8529 / % possible obs: 86 % / Observed criterion σ(I): 0 |
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Reflection | *PLUS Num. measured all: 20243 / Rmerge(I) obs: 0.053 |
Reflection shell | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 2.5 Å / % possible obs: 74 % |
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Processing
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Refinement | Resolution: 2.4→32 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.4→32 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.178 / Rfactor Rwork: 0.178 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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