+Open data
-Basic information
Entry | Database: PDB / ID: 2uy1 | ||||||
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Title | CRYSTAL STRUCTURE OF CSTF-77 | ||||||
Components | (CLEAVAGE STIMULATION FACTOR 77) x 2 | ||||||
Keywords | RNA BINDING PROTEIN / RNA-BINDING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | ENCEPHALITOZOON CUNICULI (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2 Å | ||||||
Authors | Legrand, P. / Pinaud, N. / Minvielle-Sebastia, L. / Fribourg, S. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2007 Title: The Structure of the Cstf-77 Homodimer Provides Insights Into Cstf Assembly. Authors: Legrand, P. / Pinaud, N. / Minvielle-Sebastia, L. / Fribourg, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2uy1.cif.gz | 185.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2uy1.ent.gz | 147.1 KB | Display | PDB format |
PDBx/mmJSON format | 2uy1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2uy1_validation.pdf.gz | 445.1 KB | Display | wwPDB validaton report |
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Full document | 2uy1_full_validation.pdf.gz | 463 KB | Display | |
Data in XML | 2uy1_validation.xml.gz | 34.5 KB | Display | |
Data in CIF | 2uy1_validation.cif.gz | 49.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uy/2uy1 ftp://data.pdbj.org/pub/pdb/validation_reports/uy/2uy1 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 58228.852 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ENCEPHALITOZOON CUNICULI (fungus) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q8SWC5 |
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#2: Protein | Mass: 58221.930 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ENCEPHALITOZOON CUNICULI (fungus) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q8SWC5 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 52.41 % |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-2 / Wavelength: 0.934 |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2→91 Å / Num. obs: 77953 / % possible obs: 97.9 % / Observed criterion σ(I): 2 / Redundancy: 3.7 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 15.1 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 2→91.29 Å / Cor.coef. Fo:Fc: 0.921 / Cor.coef. Fo:Fc free: 0.875 / SU B: 4.874 / SU ML: 0.139 / Cross valid method: THROUGHOUT / ESU R: 0.183 / ESU R Free: 0.178 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28 Å2
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Refinement step | Cycle: LAST / Resolution: 2→91.29 Å
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