+Open data
-Basic information
Entry | Database: PDB / ID: 2row | ||||||
---|---|---|---|---|---|---|---|
Title | The C1 domain of ROCK II | ||||||
Components | Rho-associated protein kinase 2 | ||||||
Keywords | TRANSFERASE / protein / ATP-binding / Coiled coil / Cytoplasm / Kinase / Membrane / Metal-binding / Nucleotide-binding / Phorbol-ester binding / Phosphoprotein / Serine/threonine-protein kinase / Zinc / Zinc-finger | ||||||
Function / homology | Function and homology information RHOBTB1 GTPase cycle / RHO GTPases Activate ROCKs / Sema4D induced cell migration and growth-cone collapse / VEGFA-VEGFR2 Pathway / RHOB GTPase cycle / : / cellular response to acetylcholine / RHOH GTPase cycle / G alpha (12/13) signalling events / positive regulation of connective tissue growth factor production ...RHOBTB1 GTPase cycle / RHO GTPases Activate ROCKs / Sema4D induced cell migration and growth-cone collapse / VEGFA-VEGFR2 Pathway / RHOB GTPase cycle / : / cellular response to acetylcholine / RHOH GTPase cycle / G alpha (12/13) signalling events / positive regulation of connective tissue growth factor production / positive regulation of amyloid precursor protein catabolic process / positive regulation of centrosome duplication / RHOA GTPase cycle / regulation of angiotensin-activated signaling pathway / Rho-dependent protein serine/threonine kinase activity / negative regulation of protein localization to lysosome / regulation of keratinocyte differentiation / response to transforming growth factor beta / positive regulation of connective tissue replacement / positive regulation of fibroblast growth factor production / regulation of nervous system process / regulation of cell junction assembly / negative regulation of bicellular tight junction assembly / postsynaptic actin cytoskeleton organization / regulation of protein metabolic process / regulation of synapse maturation / regulation of cellular response to hypoxia / positive regulation of protein localization to early endosome / negative regulation of nitric oxide biosynthetic process / EPHB-mediated forward signaling / embryonic morphogenesis / response to angiotensin / cellular response to testosterone stimulus / actomyosin structure organization / spindle pole centrosome / regulation of establishment of endothelial barrier / chromosome condensation / dendrite morphogenesis / cortical actin cytoskeleton organization / positive regulation of amyloid-beta formation / mRNA destabilization / positive regulation of cardiac muscle hypertrophy / centrosome duplication / Rho protein signal transduction / mitotic cytokinesis / extrinsic apoptotic signaling pathway via death domain receptors / smooth muscle contraction / epithelial to mesenchymal transition / positive regulation of stress fiber assembly / blood vessel diameter maintenance / negative regulation of angiogenesis / positive regulation of endothelial cell migration / canonical NF-kappaB signal transduction / neural tube closure / response to ischemia / protein localization to plasma membrane / Schaffer collateral - CA1 synapse / regulation of circadian rhythm / small GTPase binding / cytoplasmic ribonucleoprotein granule / rhythmic process / kinase activity / actin cytoskeleton organization / positive regulation of MAPK cascade / cytoskeleton / non-specific serine/threonine protein kinase / protein kinase activity / positive regulation of cell migration / positive regulation of protein phosphorylation / phosphorylation / negative regulation of gene expression / protein serine kinase activity / protein serine/threonine kinase activity / centrosome / glutamatergic synapse / positive regulation of gene expression / ATP binding / metal ion binding / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Wen, W. / Zhang, M. | ||||||
Citation | Journal: To be Published Title: The C1 domain of ROCK II Authors: Wen, W. / Liu, W. / Yan, J. / Zhang, M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2row.cif.gz | 533.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2row.ent.gz | 461.9 KB | Display | PDB format |
PDBx/mmJSON format | 2row.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ro/2row ftp://data.pdbj.org/pub/pdb/validation_reports/ro/2row | HTTPS FTP |
---|
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 10005.706 Da / Num. of mol.: 1 / Fragment: C1 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Rock2 / Production host: Escherichia coli (E. coli) References: UniProt: Q62868, non-specific serine/threonine protein kinase |
---|---|
#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||||||
Sample conditions | Ionic strength: 0.5 / pH: 5.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 750 MHz |
---|
-Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1 |