+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2row | ||||||
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タイトル | The C1 domain of ROCK II | ||||||
要素 | Rho-associated protein kinase 2 | ||||||
キーワード | TRANSFERASE (転移酵素) / protein (タンパク質) / ATP-binding / Coiled coil (コイルドコイル) / Cytoplasm (細胞質) / Kinase (キナーゼ) / Membrane (生体膜) / Metal-binding / Nucleotide-binding / Phorbol-ester binding / Phosphoprotein / Serine/threonine-protein kinase / Zinc (亜鉛) / Zinc-finger (ジンクフィンガー) | ||||||
機能・相同性 | 機能・相同性情報 RHOBTB1 GTPase cycle / RHO GTPases Activate ROCKs / Sema4D induced cell migration and growth-cone collapse / VEGFA-VEGFR2 Pathway / RHOB GTPase cycle / : / cellular response to acetylcholine / RHOH GTPase cycle / G alpha (12/13) signalling events / positive regulation of connective tissue growth factor production ...RHOBTB1 GTPase cycle / RHO GTPases Activate ROCKs / Sema4D induced cell migration and growth-cone collapse / VEGFA-VEGFR2 Pathway / RHOB GTPase cycle / : / cellular response to acetylcholine / RHOH GTPase cycle / G alpha (12/13) signalling events / positive regulation of connective tissue growth factor production / positive regulation of amyloid precursor protein catabolic process / positive regulation of centrosome duplication / RHOA GTPase cycle / regulation of angiotensin-activated signaling pathway / Rho-dependent protein serine/threonine kinase activity / negative regulation of protein localization to lysosome / regulation of keratinocyte differentiation / positive regulation of connective tissue replacement / positive regulation of fibroblast growth factor production / response to transforming growth factor beta / regulation of nervous system process / regulation of cell junction assembly / negative regulation of bicellular tight junction assembly / postsynaptic actin cytoskeleton organization / regulation of protein metabolic process / regulation of synapse maturation / regulation of cellular response to hypoxia / positive regulation of protein localization to early endosome / embryonic morphogenesis / EPHB-mediated forward signaling / negative regulation of nitric oxide biosynthetic process / cellular response to testosterone stimulus / actomyosin structure organization / spindle pole centrosome / regulation of establishment of endothelial barrier / response to angiotensin / chromosome condensation / dendrite morphogenesis / cortical actin cytoskeleton organization / positive regulation of amyloid-beta formation / mRNA destabilization / positive regulation of cardiac muscle hypertrophy / centrosome duplication / mitotic cytokinesis / Rho protein signal transduction / extrinsic apoptotic signaling pathway via death domain receptors / smooth muscle contraction / 上皮間葉転換 / canonical NF-kappaB signal transduction / positive regulation of stress fiber assembly / blood vessel diameter maintenance / negative regulation of angiogenesis / positive regulation of endothelial cell migration / neural tube closure / response to ischemia / protein localization to plasma membrane / Schaffer collateral - CA1 synapse / regulation of circadian rhythm / cytoplasmic ribonucleoprotein granule / small GTPase binding / rhythmic process / kinase activity / actin cytoskeleton organization / positive regulation of MAPK cascade / 細胞骨格 / non-specific serine/threonine protein kinase / protein kinase activity / positive regulation of cell migration / positive regulation of protein phosphorylation / リン酸化 / negative regulation of gene expression / protein serine kinase activity / 中心体 / protein serine/threonine kinase activity / glutamatergic synapse / positive regulation of gene expression / ATP binding / metal ion binding / 細胞核 / 細胞膜 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Rattus norvegicus (ドブネズミ) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Wen, W. / Zhang, M. | ||||||
引用 | ジャーナル: To be Published タイトル: The C1 domain of ROCK II 著者: Wen, W. / Liu, W. / Yan, J. / Zhang, M. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2row.cif.gz | 533.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2row.ent.gz | 461.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2row.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/ro/2row ftp://data.pdbj.org/pub/pdb/validation_reports/ro/2row | HTTPS FTP |
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-関連構造データ
関連構造データ | |
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類似構造データ |
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 10005.706 Da / 分子数: 1 / 断片: C1 domain / 由来タイプ: 組換発現 / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 遺伝子: Rock2 / 発現宿主: Escherichia coli (大腸菌) 参照: UniProt: Q62868, non-specific serine/threonine protein kinase |
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#2: 化合物 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料 |
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試料状態 | イオン強度: 0.5 / pH: 5.5 / 圧: ambient / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: Varian INOVA / 製造業者: Varian / モデル: INOVA / 磁場強度: 750 MHz |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 | ||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 / 代表コンフォーマー: 1 |