+Open data
-Basic information
Entry | Database: PDB / ID: 1unp | ||||||
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Title | Crystal structure of the pleckstrin homology domain of PKB alpha | ||||||
Components | RAC-ALPHA SERINE/THREONINE KINASE | ||||||
Keywords | TRANSFERASE / PLECKSTRIN HOMOLOGY DOMAIN / PHOSPHOINOSITIDE / PKB / AKT | ||||||
Function / homology | Function and homology information glycogen cell differentiation involved in embryonic placenta development / regulation of tRNA methylation / response to insulin-like growth factor stimulus / mammalian oogenesis stage / potassium channel activator activity / negative regulation of protein localization to lysosome / positive regulation of protein localization to endoplasmic reticulum / maintenance of protein location in mitochondrion / negative regulation of lymphocyte migration / cellular response to decreased oxygen levels ...glycogen cell differentiation involved in embryonic placenta development / regulation of tRNA methylation / response to insulin-like growth factor stimulus / mammalian oogenesis stage / potassium channel activator activity / negative regulation of protein localization to lysosome / positive regulation of protein localization to endoplasmic reticulum / maintenance of protein location in mitochondrion / negative regulation of lymphocyte migration / cellular response to decreased oxygen levels / maternal placenta development / regulation of type B pancreatic cell development / AKT-mediated inactivation of FOXO1A / Negative regulation of the PI3K/AKT network / negative regulation of long-chain fatty acid import across plasma membrane / establishment of protein localization to mitochondrion / activation-induced cell death of T cells / negative regulation of fatty acid beta-oxidation / regulation of glycogen biosynthetic process / AKT phosphorylates targets in the nucleus / cellular response to oxidised low-density lipoprotein particle stimulus / positive regulation of I-kappaB phosphorylation / negative regulation of cilium assembly / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / response to fluid shear stress / RUNX2 regulates genes involved in cell migration / positive regulation of organ growth / interleukin-18-mediated signaling pathway / MTOR signalling / fibroblast migration / positive regulation of sodium ion transport / mammary gland epithelial cell differentiation / negative regulation of endopeptidase activity / negative regulation of protein serine/threonine kinase activity / positive regulation of protein localization to cell surface / RAB GEFs exchange GTP for GDP on RABs / positive regulation of endodeoxyribonuclease activity / protein serine/threonine kinase inhibitor activity / positive regulation of glucose metabolic process / response to growth factor / cellular response to granulocyte macrophage colony-stimulating factor stimulus / negative regulation of leukocyte cell-cell adhesion / phosphatidylinositol-3,4-bisphosphate binding / peripheral nervous system myelin maintenance / sphingosine-1-phosphate receptor signaling pathway / glycogen biosynthetic process / positive regulation of fibroblast migration / cell migration involved in sprouting angiogenesis / response to growth hormone / anoikis / regulation of postsynapse organization / AKT phosphorylates targets in the cytosol / execution phase of apoptosis / labyrinthine layer blood vessel development / response to food / regulation of myelination / response to UV-A / KSRP (KHSRP) binds and destabilizes mRNA / Regulation of TP53 Activity through Association with Co-factors / negative regulation of macroautophagy / CTLA4 inhibitory signaling / negative regulation of cGAS/STING signaling pathway / behavioral response to pain / regulation of neuron projection development / negative regulation of Notch signaling pathway / TOR signaling / negative regulation of release of cytochrome c from mitochondria / apoptotic mitochondrial changes / Constitutive Signaling by AKT1 E17K in Cancer / non-canonical NF-kappaB signal transduction / phosphatidylinositol-3,4,5-trisphosphate binding / CD28 dependent PI3K/Akt signaling / positive regulation of cyclin-dependent protein serine/threonine kinase activity / Regulation of localization of FOXO transcription factors / carbohydrate transport / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / Activation of BAD and translocation to mitochondria / positive regulation of G1/S transition of mitotic cell cycle / positive regulation of fat cell differentiation / positive regulation of blood vessel endothelial cell migration / positive regulation of glycogen biosynthetic process / cellular response to vascular endothelial growth factor stimulus / positive regulation of lipid biosynthetic process / canonical NF-kappaB signal transduction / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / eNOS activation / Cyclin E associated events during G1/S transition / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / regulation of cell migration / Cyclin A:Cdk2-associated events at S phase entry / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / negative regulation of protein ubiquitination / lipopolysaccharide-mediated signaling pathway / cellular response to epidermal growth factor stimulus / cellular response to cadmium ion / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Regulation of TP53 Activity through Acetylation / positive regulation of TORC1 signaling / regulation of mRNA stability Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.65 Å | ||||||
Authors | Milburn, C.C. / Deak, M. / Kelly, S.M. / Price, N.C. / Alessi, D.R. / van Aalten, D.M.F. | ||||||
Citation | Journal: Biochem. J. / Year: 2003 Title: Binding of phosphatidylinositol 3,4,5-trisphosphate to the pleckstrin homology domain of protein kinase B induces a conformational change. Authors: Milburn, C.C. / Deak, M. / Kelly, S.M. / Price, N.C. / Alessi, D.R. / Van Aalten, D.M. #1: Journal: Curr.Biol. / Year: 2002 Title: High-Resolution Structure of the Pleckstrin Homology Domain of Protein Kinase B/Akt Bound to Phosphatidylinositol (3,4,5)-Trisphosphate Authors: Thomas, C.C. / Deak, M. / Alessi, D.R. / Van Aalten, M.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1unp.cif.gz | 41.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1unp.ent.gz | 28.1 KB | Display | PDB format |
PDBx/mmJSON format | 1unp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/un/1unp ftp://data.pdbj.org/pub/pdb/validation_reports/un/1unp | HTTPS FTP |
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-Related structure data
Related structure data | 1unqC 1unrC 1h10S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14542.459 Da / Num. of mol.: 1 / Fragment: PLECKSTRIN HOMOLOGY DOMAIN, RESIDUES 1-121 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PGEX4T-1 / Production host: Escherichia coli BL21 (bacteria) References: UniProt: P31749, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.75 Å3/Da / Density % sol: 30 % |
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Crystal grow | pH: 8.5 Details: 30 % PEG 4000, 0.25 M SODIUM ACETATE, 0.1 M TRIS PH 8.5 3.3 % POLYPROPYLENE GLYCOL 400 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX14.2 / Wavelength: 0.97949 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jan 15, 2002 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→30 Å / Num. obs: 12409 / % possible obs: 98.7 % / Redundancy: 3 % / Biso Wilson estimate: 17.8 Å2 / Rmerge(I) obs: 0.048 / Net I/σ(I): 4.1 |
Reflection shell | Resolution: 1.65→1.71 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.409 / Mean I/σ(I) obs: 4.1 / % possible all: 97.2 |
-Processing
Software |
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Refinement | Method to determine structure: MAD Starting model: PDB ENTRY 1H10 Resolution: 1.65→19.78 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 873989.27 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 59.3137 Å2 / ksol: 0.42143 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.65→19.78 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.65→1.75 Å / Rfactor Rfree error: 0.033 / Total num. of bins used: 6
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Xplor file |
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