Activation of NOXA and translocation to mitochondria / positive regulation of establishment of protein localization to mitochondrion / BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members / positive regulation of DNA damage response, signal transduction by p53 class mediator / positive regulation of fibroblast apoptotic process / positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway / channel activity / fibroblast apoptotic process / mitochondrial fusion / Bcl-2 family protein complex ...Activation of NOXA and translocation to mitochondria / positive regulation of establishment of protein localization to mitochondrion / BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members / positive regulation of DNA damage response, signal transduction by p53 class mediator / positive regulation of fibroblast apoptotic process / positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway / channel activity / fibroblast apoptotic process / mitochondrial fusion / Bcl-2 family protein complex / BH domain binding / T cell homeostasis / BH3 domain binding / negative regulation of mitochondrial membrane potential / intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of anoikis / response to X-ray / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / response to UV / negative regulation of fibroblast proliferation / extrinsic apoptotic signaling pathway in absence of ligand / negative regulation of autophagy / release of cytochrome c from mitochondria / response to cytokine / activation of cysteine-type endopeptidase activity involved in apoptotic process / intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of neuron apoptotic process / regulation of apoptotic process / mitochondrial outer membrane / cell differentiation / protein dimerization activity / protein heterodimerization activity / positive regulation of apoptotic process / DNA damage response / negative regulation of apoptotic process / protein-containing complex binding / apoptotic process / mitochondrion / nucleoplasm / membrane / nucleus / cytoplasm / cytosol Similarity search - Function
Phorbol-12-myristate-13-acetate-induced protein 1 / Phorbol-12-myristate-13-acetate-induced / Apoptosis regulator, Mcl-1 / Blc2-like / Apoptosis Regulator Bcl-x / Apoptosis regulator, Bcl-2, BH3 motif, conserved site / Apoptosis regulator, Bcl-2 family BH3 motif signature. / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2, BH2 motif, conserved site ...Phorbol-12-myristate-13-acetate-induced protein 1 / Phorbol-12-myristate-13-acetate-induced / Apoptosis regulator, Mcl-1 / Blc2-like / Apoptosis Regulator Bcl-x / Apoptosis regulator, Bcl-2, BH3 motif, conserved site / Apoptosis regulator, Bcl-2 family BH3 motif signature. / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Apoptosis regulator, Bcl-2 family BH2 motif signature. / BCL (B-Cell lymphoma); contains BH1, BH2 regions / Bcl-2 family / Bcl-2, Bcl-2 homology region 1-3 / Bcl2-like / Apoptosis regulator proteins, Bcl-2 family / BCL2-like apoptosis inhibitors family profile. / Bcl-2-like superfamily / Orthogonal Bundle / Mainly Alpha Similarity search - Domain/homology
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog / Phorbol-12-myristate-13-acetate-induced protein 1 Similarity search - Component
Ionic strength: 120 mM / pH: 6.7 / Pressure: ambient / Temperature: 298 K
-
NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
500
1
Bruker DRX
Bruker
DRX
600
2
Bruker Avance
Bruker
AVANCE
800
3
-
Processing
NMR software
Name
Version
Developer
Classification
TopSpin
1.3
BrukerBiospin
collection
TopSpin
1.3
BrukerBiospin
processing
XEASY
1.3
Bartelsetal.
peakpicking
XEASY
1.3
Bartelsetal.
chemicalshiftassignment
XEASY
1.3
Bartelsetal.
dataanalysis
CYANA
2.1
Guntert, MumenthalerandWuthrich
structuresolution
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
refinement
Refinement
Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 Details: The structures were calculated based on a total of 3355 constraints. 2662 are NOE derived and 184 of these are intermolecular. A total of 325 dihedral angle constraints were used and 184 hydrogen bonds.
NMR constraints
NOE constraints total: 2662 / NOE intraresidue total count: 849 / NOE long range total count: 725 / NOE medium range total count: 545 / NOE sequential total count: 543 / Hydrogen bond constraints total count: 184 / Protein phi angle constraints total count: 175 / Protein psi angle constraints total count: 150
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 256 / Conformers submitted total number: 20 / Maximum torsion angle constraint violation: 5 ° / Maximum upper distance constraint violation: 0.2 Å
NMR ensemble rms
Distance rms dev: 0.01 Å / Distance rms dev error: 0.0009 Å
+
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