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- PDB-2r63: STRUCTURAL ROLE OF A BURIED SALT BRIDGE IN THE 434 REPRESSOR DNA-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2r63 | ||||||
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Title | STRUCTURAL ROLE OF A BURIED SALT BRIDGE IN THE 434 REPRESSOR DNA-BINDING DOMAIN, NMR, 20 STRUCTURES | ||||||
![]() | REPRESSOR PROTEIN FROM BACTERIOPHAGE 434 | ||||||
![]() | GENE REGULATING PROTEIN / PHAGE 434 REPRESSOR / HELIX-TURN-HELIX / DNA-BINDING DOMAIN | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY WITH DIANA, ENERGY MINIMIZATION WITH OPAL | ||||||
![]() | Pervushin, K.V. / Billeter, M. / Siegal, G. / Wuthrich, K. | ||||||
![]() | ![]() Title: Structural role of a buried salt bridge in the 434 repressor DNA-binding domain. Authors: Pervushin, K. / Billeter, M. / Siegal, G. / Wuthrich, K. #1: ![]() Title: Determination of the Nuclear Magnetic Resonance Solution Structure of the DNA-Binding Domain (Residues 1 to 69) of the 434 Repressor and Comparison with the X-Ray Crystal Structure Authors: Neri, D. / Billeter, M. / Wuthrich, K. #2: ![]() Title: Structure of the Amino-Terminal Domain of Phage 434 Repressor at 2.0 A Resolution Authors: Mondragon, A. / Subbiah, S. / Almo, S.C. / Drottar, M. / Harrison, S.C. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 423.8 KB | Display | ![]() |
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PDB format | ![]() | 361.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 357.4 KB | Display | ![]() |
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Full document | ![]() | 460.3 KB | Display | |
Data in XML | ![]() | 18.8 KB | Display | |
Data in CIF | ![]() | 33.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 6874.874 Da / Num. of mol.: 1 / Fragment: DNA-BINDING DOMAIN, RESIDUES 1 - 63 / Mutation: R10M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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Sample preparation
Sample conditions | pH: 4.8 / Temperature: 286 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY PLUS 750 / Manufacturer: Varian / Model: UNITY PLUS 750 / Field strength: 750 MHz |
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Processing
NMR software |
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Refinement | Method: DISTANCE GEOMETRY WITH DIANA, ENERGY MINIMIZATION WITH OPAL Software ordinal: 1 | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: DIANA PENALTY FUNCTION / Conformers calculated total number: 20 / Conformers submitted total number: 20 |