[English] 日本語
![](img/lk-miru.gif)
- PDB-2qnf: Crystal structure of T4 Endonuclease VII H43N mutant in complex w... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2qnf | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of T4 Endonuclease VII H43N mutant in complex with heteroduplex DNA containing base mismatches | ||||||
![]() |
| ||||||
![]() | Hydrolase/DNA / T4 Endonuclease VII / Endo VII / resolvase / resolving-enzyme / DNA mismatch / Alternative initiation / Calcium / Hydrolase / Metal-binding / Zinc / Hydrolase-DNA COMPLEX | ||||||
Function / homology | ![]() endonuclease activity / Hydrolases; Acting on ester bonds / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Biertumpfel, C. / Yang, W. / Suck, D. | ||||||
![]() | ![]() Title: Crystal structure of T4 endonuclease VII resolving a Holliday junction. Authors: Biertumpfel, C. / Yang, W. / Suck, D. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 111.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 81.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 454.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 466.9 KB | Display | |
Data in XML | ![]() | 16.3 KB | Display | |
Data in CIF | ![]() | 21.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2qncC ![]() 1e7lS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
-DNA chain , 4 types, 4 molecules CDEF
#1: DNA chain | Mass: 4908.193 Da / Num. of mol.: 1 / Source method: obtained synthetically |
---|---|
#2: DNA chain | Mass: 4890.166 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: DNA chain | Mass: 4890.166 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#4: DNA chain | Mass: 4908.193 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Protein / Non-polymers , 2 types, 4 molecules AB![](data/chem/img/ZN.gif)
![](data/chem/img/ZN.gif)
#5: Protein | Mass: 18149.732 Da / Num. of mol.: 2 / Mutation: H43N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P13340, crossover junction endodeoxyribonuclease #6: Chemical | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.35 Å3/Da / Density % sol: 63.23 % | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 293 K / pH: 6.5 Details: 25%(w/v) PEG 3350, 100 mM Bis-Tris, 220mM Li2SO4 and 5% (w/v) glucose, pH 6.5, VAPOR DIFFUSION, temperature 293K, pH 6.50 | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 10, 2006 |
Radiation | Monochromator: SI 220. ROSENBAUM-ROCK DOUBLE -CRYSTAL MONOCHROMATOR Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→38 Å / Num. obs: 12214 / % possible obs: 84.6 % / Redundancy: 2.89 % / Biso Wilson estimate: 88.4 Å2 / Rsym value: 0.048 / Net I/σ(I): 20.8 |
Reflection shell | Resolution: 3→3.11 Å / Mean I/σ(I) obs: 5.7 / Rsym value: 0.146 / % possible all: 37.6 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1E7L Resolution: 3→37.84 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1930098.36 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER / Details: BULK SOLVENT MODEL USED
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 32.02 Å2 / ksol: 0.25 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 134.2 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→37.84 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints NCS | NCS model details: CONSTR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3→3.19 Å / Rfactor Rfree error: 0.056 / Total num. of bins used: 6
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
|