central element / meiotic spindle midzone / survivin complex / meiotic spindle midzone assembly / establishment of chromosome localization / positive regulation of mitotic sister chromatid separation / positive regulation of mitotic cytokinesis / metaphase chromosome alignment / positive regulation of exit from mitosis / positive regulation of mitotic cell cycle spindle assembly checkpoint ...central element / meiotic spindle midzone / survivin complex / meiotic spindle midzone assembly / establishment of chromosome localization / positive regulation of mitotic sister chromatid separation / positive regulation of mitotic cytokinesis / metaphase chromosome alignment / positive regulation of exit from mitosis / positive regulation of mitotic cell cycle spindle assembly checkpoint / mitotic spindle midzone assembly / positive regulation of attachment of mitotic spindle microtubules to kinetochore / chromocenter / interphase microtubule organizing center / lateral element / protein-containing complex localization / chromosome passenger complex / cysteine-type endopeptidase inhibitor activity / mitotic metaphase chromosome alignment / nuclear chromosome / intercellular bridge / mitotic spindle assembly checkpoint signaling / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / cobalt ion binding / cytoplasmic microtubule / mitotic sister chromatid segregation / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / SUMOylation of DNA replication proteins / chromosome, centromeric region / mitotic cytokinesis / mitotic spindle assembly / chromosome organization / spindle midzone / pericentric heterochromatin / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / centriole / tubulin binding / positive regulation of mitotic cell cycle / molecular function activator activity / chromosome segregation / RHO GTPases Activate Formins / sensory perception of sound / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / spindle microtubule / kinetochore / small GTPase binding / spindle / Separation of Sister Chromatids / G2/M transition of mitotic cell cycle / microtubule cytoskeleton / Neddylation / mitotic cell cycle / midbody / protein-folding chaperone binding / microtubule binding / Interleukin-4 and Interleukin-13 signaling / microtubule / nuclear body / protein heterodimerization activity / cell division / protein phosphorylation / negative regulation of DNA-templated transcription / apoptotic process / positive regulation of cell population proliferation / nucleolus / negative regulation of apoptotic process / enzyme binding / protein homodimerization activity / protein-containing complex / zinc ion binding / nucleoplasm / identical protein binding / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function
Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #3600 / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1900 / Borealin, N-terminal / Cell division protein borealin / Borealin, C-terminal / Nbl1 / Borealin N terminal / Cell division cycle-associated protein 8 / Inner centromere protein, ARK-binding domain / Chromosome passenger complex (CPC) protein INCENP N-terminal / Inner centromere protein, ARK binding region ...Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #3600 / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1900 / Borealin, N-terminal / Cell division protein borealin / Borealin, C-terminal / Nbl1 / Borealin N terminal / Cell division cycle-associated protein 8 / Inner centromere protein, ARK-binding domain / Chromosome passenger complex (CPC) protein INCENP N-terminal / Inner centromere protein, ARK binding region / Chromosome passenger complex (CPC) protein INCENP N terminal / Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A / Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A / BIR repeat / Inhibitor of Apoptosis domain / BIR repeat profile. / Baculoviral inhibition of apoptosis protein repeat / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Helix non-globular / Special / Up-down Bundle / Orthogonal Bundle / Mainly Alpha Similarity search - Domain/homology
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.99 Å / Relative weight: 1
Reflection
Resolution: 1.4→42 Å / Num. obs: 64114 / % possible obs: 96 % / Redundancy: 5.1 % / Biso Wilson estimate: 15.4 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 15.2
Reflection shell
Resolution: 1.4→1.48 Å / Redundancy: 3 % / Rmerge(I) obs: 0.392 / Mean I/σ(I) obs: 2.6 / Num. unique all: 7613 / Rsym value: 0.327 / % possible all: 80.1
-
Processing
Software
Name
Version
Classification
NB
REFMAC
5.2.0019
refinement
PDB_EXTRACT
2
dataextraction
MOSFLM
datareduction
SCALA
datascaling
SHELXS
phasing
Refinement
Method to determine structure: SAD / Resolution: 1.4→42 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.947 / SU B: 1.826 / SU ML: 0.033 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.067 / ESU R Free: 0.059 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.202
3802
5.9 %
RANDOM
Rwork
0.184
-
-
-
obs
0.186
64044
95.69 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK
Displacement parameters
Biso mean: 23.956 Å2
Baniso -1
Baniso -2
Baniso -3
1-
0.35 Å2
0 Å2
0 Å2
2-
-
-0.14 Å2
0 Å2
3-
-
-
-0.21 Å2
Refinement step
Cycle: LAST / Resolution: 1.4→42 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
2090
0
13
327
2430
Refine LS restraints
Refine-ID
Type
Dev ideal
Dev ideal target
Number
X-RAY DIFFRACTION
r_bond_refined_d
0.007
0.022
2156
X-RAY DIFFRACTION
r_angle_refined_deg
1.326
1.979
2916
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
4.422
5
264
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
34.036
24.483
116
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
12.741
15
423
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
19.407
15
18
X-RAY DIFFRACTION
r_chiral_restr
0.139
0.2
308
X-RAY DIFFRACTION
r_gen_planes_refined
0.004
0.02
1650
X-RAY DIFFRACTION
r_nbd_refined
0.203
0.2
1037
X-RAY DIFFRACTION
r_nbtor_refined
0.308
0.2
1500
X-RAY DIFFRACTION
r_xyhbond_nbd_refined
0.109
0.2
229
X-RAY DIFFRACTION
r_symmetry_vdw_refined
0.214
0.2
67
X-RAY DIFFRACTION
r_symmetry_hbond_refined
0.106
0.2
27
X-RAY DIFFRACTION
r_mcbond_it
1.013
1.5
1306
X-RAY DIFFRACTION
r_mcangle_it
1.544
2
2048
X-RAY DIFFRACTION
r_scbond_it
2.122
3
955
X-RAY DIFFRACTION
r_scangle_it
3.321
4.5
856
X-RAY DIFFRACTION
r_rigid_bond_restr
1.259
3
2261
X-RAY DIFFRACTION
r_sphericity_free
2.517
3
328
X-RAY DIFFRACTION
r_sphericity_bonded
1.738
3
2102
LS refinement shell
Resolution: 1.4→1.436 Å / Total num. of bins used: 20
Rfactor
Num. reflection
% reflection
Rfree
0.26
201
-
Rwork
0.228
3366
-
obs
-
3567
73.33 %
+
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