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Open data
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Basic information
Entry | Database: PDB / ID: 2ptg | ||||||
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Title | Crystal structure of Eimeria tenella enoyl reductase | ||||||
![]() | Enoyl-acyl carrier reductase | ||||||
![]() | OXIDOREDUCTASE / Apicomplexa / Eimeria / Eimeria tenella / enoyl (acyl-carrier-protein) reductase | ||||||
Function / homology | ![]() enoyl-[acyl-carrier-protein] reductase (NADH) activity / fatty acid biosynthetic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lu, J.Z. / Prigge, S.T. | ||||||
![]() | ![]() Title: Type I and type II fatty acid biosynthesis in Eimeria tenella: Enoyl reductase activity and structure Authors: Lu, J.Z. / Muench, S.P. / Allary, M. / Campbell, S. / Roberts, C.W. / Mui, E. / McLeod, R.L. / Rice, D.W. / Prigge, S.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96 KB | Display | ![]() |
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PDB format | ![]() | 72.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.2 KB | Display | ![]() |
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Full document | ![]() | 456.8 KB | Display | |
Data in XML | ![]() | 19.2 KB | Display | |
Data in CIF | ![]() | 25.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1enpS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33554.070 Da / Num. of mol.: 2 / Fragment: Mature form Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q0VIP6, enoyl-[acyl-carrier-protein] reductase (NADH) |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.44 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 50mM Sodium Citrate (pH5), 5% (v/v) polyethylene glycol 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: BRUKER SMART 2000 / Detector: CCD / Date: Apr 15, 2005 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. all: 18684 / Num. obs: 18683 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.8 % / Biso Wilson estimate: 37.4 Å2 / Rmerge(I) obs: 0.09 / Rsym value: 0.107 / Net I/σ(I): 17.7 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.539 / Mean I/σ(I) obs: 2.2 / Num. unique all: 1840 / Rsym value: 0.598 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: Brassica napus ENR (1ENP) Resolution: 2.6→27.68 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 346831.6 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 1 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 72.6345 Å2 / ksol: 0.426143 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 51.7 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.6→27.68 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.69 Å / Rfactor Rfree error: 0.037 / Total num. of bins used: 10
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Xplor file | Serial no: 1 / Param file: protein_rep.param / Topol file: protein.top |