- PDB-2ps9: Structure and metal binding properties of ZnuA, a periplasmic zin... -
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Basic information
Entry
Database: PDB / ID: 2ps9
Title
Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli
Components
High-affinity zinc uptake system protein znuA
Keywords
METAL TRANSPORT / two-domain protein / each domain has (beta/alfa)4 fold with cobalt binding cleft between two domains
Function / homology
Function and homology information
zinc ion import across plasma membrane / zinc ion transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / periplasmic space / cell adhesion / zinc ion binding / membrane Similarity search - Function
High-affinity zinc uptake system protein ZnuA / Adhesion lipoprotein / Periplasmic solute binding protein, ZnuA-like / Zinc-uptake complex component A periplasmic / Nitrogenase molybdenum iron protein domain / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 291 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.45 Å3/Da / Density % sol: 49.7 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 7.25 mg/mL CoZnuA in buffer H (50 mM HEPES, pH 7.5, 200 mM NaCl) was mixed 1:1 with 25 % (w/v) PEG 4000, 3.5 % isopropanol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
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Data collection
Diffraction
ID
Mean temperature (K)
Crystal-ID
1
100
1
2
1
3
1
Diffraction source
Source
Site
Beamline
ID
Wavelength
SYNCHROTRON
APS
5ID-B
1
0.97934
SYNCHROTRON
APS
23-ID-D
2
0.97934
SYNCHROTRON
APS
19-ID
3
0.97934
Detector
Type: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: Jul 6, 2006 / Details: mirrors
Radiation
Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.97934 Å / Relative weight: 1
Reflection
Redundancy: 6.5 % / Av σ(I) over netI: 10.7 / Number: 210028 / Rmerge(I) obs: 0.086 / Χ2: 1.01 / D res high: 2.15 Å / D res low: 50 Å / Num. obs: 32326 / % possible obs: 95.4
Diffraction reflection shell
Highest resolution (Å)
Lowest resolution (Å)
% possible obs (%)
ID
Rmerge(I) obs
Chi squared
Redundancy
4.63
50
99.2
1
0.044
1.004
6.9
3.68
4.63
100
1
0.056
0.99
7.2
3.21
3.68
100
1
0.081
1.013
7.4
2.92
3.21
100
1
0.119
1.003
7.4
2.71
2.92
100
1
0.183
1.003
7.3
2.55
2.71
99.6
1
0.245
1.046
6.8
2.42
2.55
97.5
1
0.286
1.035
6.1
2.32
2.42
92.1
1
0.307
1.022
5.4
2.23
2.32
86.5
1
0.366
1.001
5
2.15
2.23
78.6
1
0.372
1.002
4.7
Reflection
Resolution: 2.15→50 Å / Num. obs: 32326 / % possible obs: 95.4 % / Redundancy: 6.5 % / Biso Wilson estimate: 39.4 Å2 / Rmerge(I) obs: 0.086 / Rsym value: 0.086 / Χ2: 1.012 / Net I/σ(I): 10.7
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