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- PDB-5uyf: YfeA from M9 minimal media supplemented with zinc - Mn wavelength -
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Open data
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Basic information
Entry | Database: PDB / ID: 5uyf | ||||||
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Title | YfeA from M9 minimal media supplemented with zinc - Mn wavelength | ||||||
![]() | Periplasmic chelated iron-binding protein YfeA | ||||||
![]() | METAL TRANSPORT / polyspecific / transition metal / transport | ||||||
Function / homology | ![]() cellular response to iron ion / iron ion transport / periplasmic space / cell adhesion / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Radka, C.D. / DeLucas, L.J. / Aller, S.G. | ||||||
![]() | ![]() Title: Crystal structure of Yersinia pestis virulence factor YfeA reveals two polyspecific metal-binding sites. Authors: Radka, C.D. / DeLucas, L.J. / Wilson, L.S. / Lawrenz, M.B. / Perry, R.D. / Aller, S.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.8 KB | Display | ![]() |
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PDB format | ![]() | 51.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5uxsC ![]() 5uxuC ![]() 5uy0C ![]() 5uy4C ![]() 5uy5C ![]() 5uyaC ![]() 5uybC ![]() 5uycC ![]() 5uydSC ![]() 5uyeC ![]() 5uygC ![]() 5uyhC ![]() 5uyvC ![]() 5uywC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 36048.809 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-MN / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.71 Å3/Da / Density % sol: 28 % / Description: tetragonal prism |
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Crystal grow | Temperature: 295.15 K / Method: vapor diffusion / pH: 6.3 Details: 30% PEG 4000, 20mM Bis-tris propane, 50mM sodium chloride, 0.05% w/v sodium azide |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: Jan 10, 2017 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.89289 Å / Relative weight: 1 |
Reflection | Resolution: 2.057→50 Å / Num. obs: 15986 / % possible obs: 99.9 % / Redundancy: 7.2 % / Biso Wilson estimate: 34.315 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.093 / Rpim(I) all: 0.037 / Net I/σ(I): 67.3 |
Reflection shell | Resolution: 2.057→2.1 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.411 / Mean I/σ(I) obs: 13.5 / Num. unique obs: 800 / CC1/2: 0.883 / Rpim(I) all: 0.171 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5uyd Resolution: 2.057→38.3 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 19.5
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.057→38.3 Å
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Refine LS restraints |
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LS refinement shell |
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