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Yorodumi- PDB-2nt4: Receiver domain from Myxococcus xanthus social motility protein F... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2nt4 | ||||||
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Title | Receiver domain from Myxococcus xanthus social motility protein FrzS (H92F mutant) | ||||||
Components | Response regulator homolog | ||||||
Keywords | SIGNALING PROTEIN / social motility / signaling / receiver domain / two-component | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Myxococcus xanthus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.02 Å | ||||||
Authors | Echols, N. / Fraser, J. / Weisfield, S. / Merlie, J. / Zusman, D. / Alber, T. | ||||||
Citation | Journal: Mol.Microbiol. / Year: 2007 Title: An atypical receiver domain controls the dynamic polar localization of the Myxococcus xanthus social motility protein FrzS. Authors: Fraser, J.S. / Merlie, J.P. / Echols, N. / Weisfield, S.R. / Mignot, T. / Wemmer, D.E. / Zusman, D.R. / Alber, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2nt4.cif.gz | 76.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2nt4.ent.gz | 56.4 KB | Display | PDB format |
PDBx/mmJSON format | 2nt4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nt/2nt4 ftp://data.pdbj.org/pub/pdb/validation_reports/nt/2nt4 | HTTPS FTP |
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-Related structure data
Related structure data | 2gkgSC 2i6fC 2nt3C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13695.551 Da / Num. of mol.: 1 / Fragment: Receiver domain / Mutation: H92F Source method: isolated from a genetically manipulated source Source: (gene. exp.) Myxococcus xanthus (bacteria) / Gene: frzS / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21+ / References: UniProt: O68522 |
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#2: Chemical | ChemComp-CL / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.68 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 100mM Bis-Tris pH 5.5, 25% PEG 3350, 20% MPD added before freezing, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 0.8856 |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Mar 12, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8856 Å / Relative weight: 1 |
Reflection | Resolution: 1.02→20 Å / Num. all: 51807 / Num. obs: 51807 / % possible obs: 96.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Biso Wilson estimate: 6.5 Å2 / Rmerge(I) obs: 0.043 / Rsym value: 0.043 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 1.02→1.08 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.327 / Mean I/σ(I) obs: 2 / Num. unique all: 6553 / Rsym value: 0.327 / % possible all: 82.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: pdb entry 2gkg Resolution: 1.02→20 Å / Cor.coef. Fo:Fc: 0.982 / Cor.coef. Fo:Fc free: 0.971 / SU B: 0.694 / SU ML: 0.016 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / ESU R: 0.024 / ESU R Free: 0.027 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 8.627 Å2
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Refinement step | Cycle: LAST / Resolution: 1.02→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.02→1.075 Å / Total num. of bins used: 10
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