+Open data
-Basic information
Entry | Database: PDB / ID: 2n0z | |||||||||
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Title | Solution structure of MyUb (1080-1122) of human Myosin VI | |||||||||
Components | Unconventional myosin-VI | |||||||||
Keywords | MOTOR PROTEIN / PROTEIN TRANSPORT | |||||||||
Function / homology | Function and homology information regulation of secretion / minus-end directed microfilament motor activity / unconventional myosin complex / actin filament-based movement / clathrin-coated vesicle membrane / inner ear auditory receptor cell differentiation / Gap junction degradation / vesicle transport along actin filament / Trafficking of AMPA receptors / RHOBTB1 GTPase cycle ...regulation of secretion / minus-end directed microfilament motor activity / unconventional myosin complex / actin filament-based movement / clathrin-coated vesicle membrane / inner ear auditory receptor cell differentiation / Gap junction degradation / vesicle transport along actin filament / Trafficking of AMPA receptors / RHOBTB1 GTPase cycle / microfilament motor activity / inner ear morphogenesis / filamentous actin / microvillus / cytoskeletal motor activity / RHOU GTPase cycle / endocytic vesicle / DNA damage response, signal transduction by p53 class mediator / autophagosome / RHOBTB2 GTPase cycle / clathrin-coated pit / ruffle / filopodium / actin filament organization / actin filament / ADP binding / sensory perception of sound / intracellular protein transport / ruffle membrane / endocytosis / actin filament binding / actin cytoskeleton / apical part of cell / actin binding / cell cortex / cytoplasmic vesicle / nuclear membrane / calmodulin binding / endosome / response to xenobiotic stimulus / lysosomal membrane / perinuclear region of cytoplasm / Golgi apparatus / extracellular exosome / nucleoplasm / ATP binding / membrane / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Model details | lowest energy, model1 | |||||||||
Authors | He, F. / Walters, K. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Cell Rep / Year: 2016 Title: Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains. Authors: He, F. / Wollscheid, H.P. / Nowicka, U. / Biancospino, M. / Valentini, E. / Ehlinger, A. / Acconcia, F. / Magistrati, E. / Polo, S. / Walters, K.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n0z.cif.gz | 324.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n0z.ent.gz | 271.4 KB | Display | PDB format |
PDBx/mmJSON format | 2n0z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n0/2n0z ftp://data.pdbj.org/pub/pdb/validation_reports/n0/2n0z | HTTPS FTP |
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-Related structure data
Related structure data | 2n10C 2n11C 2n13C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5922.730 Da / Num. of mol.: 1 / Fragment: residues 1080-1122 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MYO6 / Production host: Escherichia coli (E. coli) / References: UniProt: Q5JVM0, UniProt: Q9UM54*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 0.3 mM [U-13C; U-15N] Unconventional myosin-VI, 90% H2O/10% D2O Label: sample / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.3 mM / Component: Unconventional myosin-VI / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 50 mM / Label: conditions / pH: 6.5 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 2 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 98 / Conformers submitted total number: 20 |