+Open data
-Basic information
Entry | Database: PDB / ID: 1pid | ||||||
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Title | BOVINE DESPENTAPEPTIDE INSULIN | ||||||
Components | (DESPENTAPEPTIDE INSULIN) x 2 | ||||||
Keywords | HORMONE / GLUCOSE METABOLISM | ||||||
Function / homology | Function and homology information estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate ...estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate / feeding behavior / response to growth hormone / response to food / positive regulation of Rho protein signal transduction / positive regulation of peptide hormone secretion / protein secretion / negative regulation of lipid catabolic process / response to glucose / response to nutrient levels / positive regulation of protein secretion / insulin receptor binding / positive regulation of insulin secretion / hormone activity / glucose metabolic process / glucose homeostasis / response to heat / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of gene expression / negative regulation of apoptotic process / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.3 Å | ||||||
Authors | Holden, P.H. / Papiz, M. / Dodson, G.G. | ||||||
Citation | Journal: Proteins / Year: 1997 Title: A model of insulin fibrils derived from the x-ray crystal structure of a monomeric insulin (despentapeptide insulin). Authors: Brange, J. / Dodson, G.G. / Edwards, D.J. / Holden, P.H. / Whittingham, J.L. #1: Journal: Acta Crystallogr.,Sect.B / Year: 1983 Title: Molecular Replacement Studies on Crystal Forms of Despentapeptide Insulin Authors: Bi, R.-C. / Cutfield, S.M. / Dodson, E.J. / Dodson, G.G. / Giordano, F. / Reynolds, C.D. / Tolley, S.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pid.cif.gz | 29.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pid.ent.gz | 21.5 KB | Display | PDB format |
PDBx/mmJSON format | 1pid.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1pid_validation.pdf.gz | 366.1 KB | Display | wwPDB validaton report |
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Full document | 1pid_full_validation.pdf.gz | 370.5 KB | Display | |
Data in XML | 1pid_validation.xml.gz | 3.6 KB | Display | |
Data in CIF | 1pid_validation.cif.gz | 5.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pi/1pid ftp://data.pdbj.org/pub/pdb/validation_reports/pi/1pid | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.99439, 0.035258, 0.099728), Vector: |
-Components
#1: Protein/peptide | Mass: 2339.645 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 #2: Protein/peptide | Mass: 2842.277 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.72 Å3/Da / Density % sol: 28.43 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: batch method | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Num. obs: 31367 / % possible obs: 90.7 % |
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.3→25.4 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Resolution: 1.3→25.4 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.19 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |