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- PDB-2n0j: Solution NMR Structure of the 27 nucleotide engineered neomycin s... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2n0j | ||||||
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Title | Solution NMR Structure of the 27 nucleotide engineered neomycin sensing riboswitch RNA-ribostamycin complex | ||||||
![]() | RNA_(27-MER) | ||||||
![]() | RNA / Riboswitches / RNA-ligand interaction / Aminoglycosides / RNA recognition / RNA structure | ||||||
Function / homology | RIBOSTAMYCIN / RNA / RNA (> 10)![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | ||||||
Model details | closest to the average, model5 | ||||||
![]() | Duchardt-Ferner, E. / Gottstein-Schmidtke, S.R. / Weigand, J.E. / Ohlenschlaeger, O.E. / Wurm, J. / Hammann, C. / Suess, B. / Woehnert, J. | ||||||
![]() | ![]() Title: What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch. Authors: Duchardt-Ferner, E. / Gottstein-Schmidtke, S.R. / Weigand, J.E. / Ohlenschlager, O. / Wurm, J.P. / Hammann, C. / Suess, B. / Wohnert, J. #1: Journal: Biomol.Nmr Assign. / Year: 2010 Title: NMR resonance assignments of an engineered neomycin-sensing riboswitch RNA bound to ribostamycin and tobramycin. Authors: Schmidtke, S.R. / Duchardt-Ferner, E. / Weigand, J.E. / Suess, B. / Wohnert, J. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 361.7 KB | Display | ![]() |
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PDB format | ![]() | 300.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 463.6 KB | Display | ![]() |
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Full document | ![]() | 579.7 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 19.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2mxsC C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 8557.031 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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#2: Chemical | ChemComp-RIO / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 879 / NOE interentity total count: 103 / NOE intraresidue total count: 429 / NOE long range total count: 127 / NOE sequential total count: 220 / Hydrogen bond constraints total count: 50 | ||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 / Maximum lower distance constraint violation: 0 Å / Maximum upper distance constraint violation: 0 Å |