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- PDB-1bnr: BARNASE -

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Basic information

Entry
Database: PDB / ID: 1bnr
TitleBARNASE
ComponentsBARNASE (G SPECIFIC ENDONUCLEASE)
KeywordsMICROBIAL RIBONUCLEASE
Function / homology
Function and homology information


Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / RNA endonuclease activity / RNA binding / extracellular region
Similarity search - Function
: / Barnase / Microbial ribonucleases / Guanine-specific ribonuclease N1/T1/U2 / Ribonuclease/ribotoxin / ribonuclease / Nuclear Transport Factor 2; Chain: A, / Roll / Alpha Beta
Similarity search - Domain/homology
Biological speciesBacillus amyloliquefaciens (bacteria)
MethodSOLUTION NMR
AuthorsBycroft, M.
CitationJournal: Biochemistry / Year: 1991
Title: Determination of the three-dimensional solution structure of barnase using nuclear magnetic resonance spectroscopy.
Authors: Bycroft, M. / Ludvigsen, S. / Fersht, A.R. / Poulsen, F.M.
History
DepositionMar 31, 1995Processing site: BNL
Revision 1.0Jul 31, 1995Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: BARNASE (G SPECIFIC ENDONUCLEASE)


Theoretical massNumber of molelcules
Total (without water)12,3991
Polymers12,3991
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / -
Representative

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Components

#1: Protein BARNASE (G SPECIFIC ENDONUCLEASE)


Mass: 12398.721 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: NMR, 20 STRUCTURES / Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / Gene: BARNASE / Plasmid: PTZ18 DERIVED / Gene (production host): BARNASE / Production host: Escherichia coli (E. coli)
References: UniProt: P00648, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameVersionClassification
X-PLOR3model building
X-PLOR3refinement
X-PLOR3phasing
NMR softwareName: X-PLOR / Version: 3 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 20

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