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Open data
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Basic information
Entry | Database: PDB / ID: 1etj | ||||||
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Title | AZURIN MUTANT WITH MET 121 REPLACED BY GLU | ||||||
![]() | AZURIN | ||||||
![]() | ELECTRON TRANSPORT / COPPER / PERIPLASMIC | ||||||
Function / homology | ![]() transition metal ion binding / periplasmic space / electron transfer activity / copper ion binding / zinc ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Karlsson, B.G. / Tsai, L.-C. / Nar, H. / Sanders-Loehr, J. / Bonander, N. / Langer, V. / Sjolin, L. | ||||||
![]() | ![]() Title: X-ray structure determination and characterization of the Pseudomonas aeruginosa azurin mutant Met121Glu. Authors: Karlsson, B.G. / Tsai, L.C. / Nar, H. / Sanders-Loehr, J. / Bonander, N. / Langer, V. / Sjolin, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 113.7 KB | Display | ![]() |
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PDB format | ![]() | 88.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 384.3 KB | Display | ![]() |
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Full document | ![]() | 390.7 KB | Display | |
Data in XML | ![]() | 11.2 KB | Display | |
Data in CIF | ![]() | 19.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13959.717 Da / Num. of mol.: 4 / Mutation: M121E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | ChemComp-CU / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 55 % | ||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6 Details: THE BLUISH WELL-FORMED PRISMATIC CRYSTALS OF THE TITLE PROTEIN WERE OBTAINED BY THE VAPOR-DIFFUSION HANGING-DROP TECHNIQUE FROM A SOLUTION CONTAINING 25% PEG4000, 0.24M CALCIUM DICHLORIDE ...Details: THE BLUISH WELL-FORMED PRISMATIC CRYSTALS OF THE TITLE PROTEIN WERE OBTAINED BY THE VAPOR-DIFFUSION HANGING-DROP TECHNIQUE FROM A SOLUTION CONTAINING 25% PEG4000, 0.24M CALCIUM DICHLORIDE AND 0.26M LITHIUM NITRATE BUFFER AT PH 6.0 AND AT THE TEMPERATURE OF 24 - 25 CENTIGRADE IN AROUND 10 DAYS., vapor diffusion - hanging drop | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() |
Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→8 Å / Num. obs: 17964 / % possible obs: 75.7 % / Observed criterion σ(I): 2 / Redundancy: 2.77 % / Rmerge(I) obs: 0.115 / Rsym value: 0.115 |
Reflection | *PLUS Num. measured all: 47678 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: WILD TYPE AZURIN MONOMER Rfactor Rwork: 0.184 / Rfactor obs: 0.184 / Highest resolution: 2.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.3 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 8 Å / Num. reflection obs: 15836 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |