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- PDB-2mz2: Phosphorylation of CB1Cannabinoid Receptor Fourth Intracellular L... -

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Basic information

Entry
Database: PDB / ID: 2mz2
TitlePhosphorylation of CB1Cannabinoid Receptor Fourth Intracellular Loop Peptides: Effects on Structure and Function
ComponentsCannabinoid receptor 1
KeywordsMembrane Protein / Signaling Protein / Intracellular loop / Phosphorylation / helix / G Protein coupled receptor / protein kinase C
Function / homology
Function and homology information


cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / negative regulation of serotonin secretion ...cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / negative regulation of serotonin secretion / regulation of feeding behavior / regulation of presynaptic cytosolic calcium ion concentration / negative regulation of action potential / Class A/1 (Rhodopsin-like receptors) / positive regulation of blood pressure / positive regulation of fever generation / regulation of metabolic process / axonal fasciculation / regulation of synaptic transmission, GABAergic / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / maternal process involved in female pregnancy / GABA-ergic synapse / regulation of synaptic transmission, glutamatergic / negative regulation of blood pressure / regulation of insulin secretion / response to nutrient / response to cocaine / response to nicotine / G protein-coupled receptor activity / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / memory / positive regulation of neuron projection development / actin cytoskeleton / glucose homeostasis / presynaptic membrane / growth cone / G alpha (i) signalling events / spermatogenesis / response to ethanol / mitochondrial outer membrane / response to lipopolysaccharide / positive regulation of apoptotic process / membrane raft / glutamatergic synapse / identical protein binding / plasma membrane / cytoplasm
Similarity search - Function
Cannabinoid receptor type 1 / Cannabinoid receptor family / Serpentine type 7TM GPCR chemoreceptor Srsx / G-protein coupled receptors family 1 signature. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / 7 transmembrane receptor (rhodopsin family)
Similarity search - Domain/homology
Cannabinoid receptor 1
Similarity search - Component
MethodSOLUTION NMR / torsion angle dynamics
Model detailsclosest to the average, model1
AuthorsGanjiwale, A. / Eldeeb, K. / Chandrashekar, I. / Cowsik, S. / Howlett, A.
CitationJournal: To be Published
Title: Phosphorylation of CB1Cannabinoid Receptor Fourth Intracellular Loop Peptides: Effects on Structure and Function
Authors: Ganjiwale, A. / Eldeeb, K. / Chandrashekar, I. / Cowsik, S. / Howlett, A.
History
DepositionFeb 5, 2015Deposition site: BMRB / Processing site: RCSB
Revision 1.0Feb 17, 2016Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Cannabinoid receptor 1


Theoretical massNumber of molelcules
Total (without water)2,1361
Polymers2,1361
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 50target function
RepresentativeModel #1closest to the average

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Components

#1: Protein/peptide Cannabinoid receptor 1 / CB-R / CB1 / CANN6


Mass: 2136.309 Da / Num. of mol.: 1 / Fragment: residues 400-414 / Source method: obtained synthetically / Details: Custom synthesis / References: UniProt: P21554*PLUS

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-1H TOCSY
1212D 1H-1H NOESY

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Sample preparation

DetailsContents: 5 mM IL4pSER402 peptide, 72 mM [U-100% 2H] SDS, 90% H2O/10% D2O
Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)ComponentIsotopic labelingSolution-ID
5 mMIL4pSER402 peptide-11
72 mMSDS-2[U-100% 2H]1
Sample conditionsIonic strength: 5 / pH: 4.5 / Pressure: ambient / Temperature: 310 K

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NMR measurement

NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz

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Processing

NMR softwareName: CYANA / Developer: Guntert P. / Classification: refinement
RefinementMethod: torsion angle dynamics / Software ordinal: 1
NMR representativeSelection criteria: closest to the average
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 50 / Conformers submitted total number: 20

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