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Yorodumi- PDB-2mz3: Phosphorylation of CB1Cannabinoid Receptor Fourth Intracellular L... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mz3 | ||||||
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Title | Phosphorylation of CB1Cannabinoid Receptor Fourth Intracellular Loop Pepducins: Effects on Structure and Function | ||||||
Components | Cannabinoid receptor 1 | ||||||
Keywords | Membrane Protein / Signaling Protein / Phosphorylation / Protein Kinase C / G Protein coupled receptor / Bradykinin | ||||||
Function / homology | Function and homology information cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / negative regulation of serotonin secretion ...cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / negative regulation of serotonin secretion / regulation of feeding behavior / regulation of presynaptic cytosolic calcium ion concentration / negative regulation of action potential / Class A/1 (Rhodopsin-like receptors) / positive regulation of blood pressure / positive regulation of fever generation / regulation of metabolic process / axonal fasciculation / regulation of synaptic transmission, GABAergic / regulation of insulin secretion / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / GABA-ergic synapse / maternal process involved in female pregnancy / response to nutrient / regulation of synaptic transmission, glutamatergic / negative regulation of blood pressure / response to cocaine / G protein-coupled receptor activity / response to nicotine / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / memory / positive regulation of neuron projection development / glucose homeostasis / actin cytoskeleton / presynaptic membrane / G alpha (i) signalling events / growth cone / spermatogenesis / response to ethanol / mitochondrial outer membrane / response to lipopolysaccharide / membrane raft / positive regulation of apoptotic process / glutamatergic synapse / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | closest to the average, model1 | ||||||
Authors | Ganjiwale, A. / Eldeeb, K. / Chandrashekaran, I. / Howlett, A. / Cowsik, S. | ||||||
Citation | Journal: To be Published Title: Phosphorylation of CB1Cannabinoid Receptor Fourth Intracellular Loop Pepducins: Effects on Structure and Function Authors: Ganjiwale, A. / Eldeeb, K. / Chandrashekaran, I. / Cowsik, S. / Howlett, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mz3.cif.gz | 117.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mz3.ent.gz | 94.3 KB | Display | PDB format |
PDBx/mmJSON format | 2mz3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mz3_validation.pdf.gz | 385.7 KB | Display | wwPDB validaton report |
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Full document | 2mz3_full_validation.pdf.gz | 457.2 KB | Display | |
Data in XML | 2mz3_validation.xml.gz | 8.1 KB | Display | |
Data in CIF | 2mz3_validation.cif.gz | 11.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mz/2mz3 ftp://data.pdbj.org/pub/pdb/validation_reports/mz/2mz3 | HTTPS FTP |
-Related structure data
Related structure data | 2mzaC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2136.309 Da / Num. of mol.: 1 / Fragment: residues 400-414 / Source method: obtained synthetically / Details: Custom synthesis / References: UniProt: P21554*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 5 mM IL4pSER415 peptide, 72 mM [U-100% 2H] SDS, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 5 / pH: 4.5 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR software | Name: CYANA / Classification: refinement |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 50 / Conformers submitted total number: 20 |