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- PDB-2mqt: Solution NMR structure of the U5-primer binding site (U5-PBS) dom... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2mqt | ||||||
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Title | Solution NMR structure of the U5-primer binding site (U5-PBS) domain of murine leukemia virus RNA genome | ||||||
![]() | RNA (68-MER) | ||||||
![]() | RNA / U5-primer binding site / U5-PBS / YNMG tetraloop | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Model details | lowest energy, model1 | ||||||
![]() | D'Souza, V. / Yildiz, Z. | ||||||
![]() | ![]() Title: A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing. Authors: Miller, S.B. / Yildiz, F.Z. / Lo, J.A. / Wang, B. / D'Souza, V.M. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 424.1 KB | Display | ![]() |
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PDB format | ![]() | 356.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 364.3 KB | Display | ![]() |
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Full document | ![]() | 439.5 KB | Display | |
Data in XML | ![]() | 14.2 KB | Display | |
Data in CIF | ![]() | 24.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2mqvC ![]() 2ms0C ![]() 2ms1C C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 21893.887 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: in vitro transcription by T7 polymerase using pUC19 vector Source: (synth.) ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.8 mM [U-100% 13C; U-100% 15N] U5-PBS, 100% D2O / Solvent system: 100% D2O |
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Sample | Conc.: 0.8 mM / Component: U5-PBS-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 10mM Tris, 10mM NaCl / pH: 7.2 / Pressure: ambient / Temperature: 311 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 700 MHz |
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Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 20 / Conformers submitted total number: 10 |