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Yorodumi- PDB-2mma: NMR-based docking model of GrxS14-BolA2 apo-heterodimer from Arab... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mma | ||||||
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Title | NMR-based docking model of GrxS14-BolA2 apo-heterodimer from Arabidopsis thaliana | ||||||
Components |
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Keywords | GENE REGULATION / stress-responsive protein / transcriptional regulator | ||||||
Function / homology | Function and homology information protein maturation by iron-sulfur cluster transfer / chloroplast envelope / antiporter activity / response to iron ion / chloroplast stroma / monoatomic cation transport / chloroplast / 2 iron, 2 sulfur cluster binding / response to oxidative stress / metal ion binding ...protein maturation by iron-sulfur cluster transfer / chloroplast envelope / antiporter activity / response to iron ion / chloroplast stroma / monoatomic cation transport / chloroplast / 2 iron, 2 sulfur cluster binding / response to oxidative stress / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | SOLUTION NMR / molecular dynamics, energy minimization | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Roret, T. / Tsan, P. / Couturier, J. / Rouhier, N. / Didierjean, C. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2014 Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes. Authors: Roret, T. / Tsan, P. / Couturier, J. / Zhang, B. / Johnson, M.K. / Rouhier, N. / Didierjean, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mma.cif.gz | 66.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mma.ent.gz | 54 KB | Display | PDB format |
PDBx/mmJSON format | 2mma.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mm/2mma ftp://data.pdbj.org/pub/pdb/validation_reports/mm/2mma | HTTPS FTP |
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-Related structure data
Related structure data | 2mm9C 4pugC 4puhC 4puiC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12354.149 Da / Num. of mol.: 1 / Fragment: UNP residues 67-173 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: GRXS14, CXIP1, At3g54900, F28P10.120 / Plasmid: pET15b / Production host: Escherichia coli (E. coli) / References: UniProt: Q84Y95 |
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#2: Protein | Mass: 10258.644 Da / Num. of mol.: 1 / Fragment: UNP residues 2-81 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: AT5G09830, At5g09830 / Plasmid: pET3d / Production host: Escherichia coli (E. coli) / References: UniProt: Q9FIC3 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.05 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics, energy minimization / Software ordinal: 1 Details: Energy minimization using the YASARA force field. The structure was moved to a nearby (local) minimum, with removal of clashes and small errors. | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 1 / Conformers submitted total number: 1 |