+Open data
-Basic information
Entry | Database: PDB / ID: 2mm9 | ||||||
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Title | Solution structure of reduced BolA2 from Arabidopsis thaliana | ||||||
Components | BolA2 | ||||||
Keywords | TRANSCRIPTION / stress-responsive protein / transcriptional regulator / morphogen | ||||||
Function / homology | Function and homology information protein maturation by iron-sulfur cluster transfer / response to iron ion / 2 iron, 2 sulfur cluster binding / intracellular iron ion homeostasis / response to oxidative stress / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Model details | minimized average structure, model1 | ||||||
Model type details | minimized average | ||||||
Authors | Roret, T. / Tsan, P. / Couturier, J. / Rouhier, N. / Didierjean, C. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2014 Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes. Authors: Roret, T. / Tsan, P. / Couturier, J. / Zhang, B. / Johnson, M.K. / Rouhier, N. / Didierjean, C. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: AUTHOR DETERMINED |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mm9.cif.gz | 567.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mm9.ent.gz | 476.2 KB | Display | PDB format |
PDBx/mmJSON format | 2mm9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mm9_validation.pdf.gz | 408.9 KB | Display | wwPDB validaton report |
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Full document | 2mm9_full_validation.pdf.gz | 532.9 KB | Display | |
Data in XML | 2mm9_validation.xml.gz | 33.1 KB | Display | |
Data in CIF | 2mm9_validation.cif.gz | 54.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mm/2mm9 ftp://data.pdbj.org/pub/pdb/validation_reports/mm/2mm9 | HTTPS FTP |
-Related structure data
Related structure data | 2mmaC 4pugC 4puhC 4puiC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10389.839 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: AT5G09830, At5g09830 / Plasmid: pET3d / Production host: Escherichia coli (E. coli) / References: UniProt: Q9FIC3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.05 / pH: 6 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |