+Open data
-Basic information
Entry | Database: PDB / ID: 2j4m | ||||||
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Title | Double dockerin from Piromyces equi Cel45A | ||||||
Components | ENDOGLUCANASE 45A | ||||||
Keywords | PROTEIN BINDING / DOCKERIN / CELLULOSOME / SMALL CYSTEINE-RICH | ||||||
Function / homology | Function and homology information | ||||||
Biological species | PIROMYCES EQUI (fungus) | ||||||
Method | SOLUTION NMR / CNS | ||||||
Authors | Nagy, T. / Tunnicliffe, R.B. / Higgins, L.D. / Walters, C. / Gilbert, H.J. / Williamson, M.P. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: Characterization of a Double Dockerin from the Cellulosome of the Anaerobic Fungus Piromyces Equi. Authors: Nagy, T. / Tunnicliffe, R.B. / Higgins, L.D. / Walters, C. / Gilbert, H.J. / Williamson, M.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2j4m.cif.gz | 171.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2j4m.ent.gz | 141.6 KB | Display | PDB format |
PDBx/mmJSON format | 2j4m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j4/2j4m ftp://data.pdbj.org/pub/pdb/validation_reports/j4/2j4m | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10910.516 Da / Num. of mol.: 1 / Fragment: FIRST TWO DOCKERIN DOMAINS, RESIDUES 21-118 Source method: isolated from a genetically manipulated source Source: (gene. exp.) PIROMYCES EQUI (fungus) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q9P868 |
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Sequence details | N-TERMINAL MET AND C-TERMINAL CLONING ARTIFACTS |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: TRIPLE RESONANCE SUITE |
NMR details | Text: MODEL 1 IS A MINIMIZED AVERAGE. THESE STRUCTURES ARE THE MAJOR CONFORMER SEEN IN THE OVERALL ENSEMBLE, WHEREAS PDB 2J4N ARE THE MINOR CONFORMER |
-Sample preparation
Details | Contents: 90% WATER/10% D2O |
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Sample conditions | Ionic strength: 50 mM / pH: 6.5 / Pressure: 1.0 atm / Temperature: 298.0 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: CNS / Software ordinal: 1 / Details: STANDARD METHODS | ||||||||||||
NMR ensemble | Conformer selection criteria: RANDOM FROM BEST 25 / Conformers calculated total number: 80 / Conformers submitted total number: 6 |