Mass: 9043.641 Da / Num. of mol.: 1 / Fragment: UNP B1LBP1 RESIDUES 3-45, 68-102 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermotoga sp. (bacteria) / Strain: RQ2 / Gene: rpsJ, TRQ2_1395 / Production host: Escherichia coli (E. coli) / References: UniProt: B1LBP1
Sequence details
RIBOSOMAL BINDING LOOP (WILD TYPE SEQUENCE REGION 46-67) REPLACED BY A SINGLE SERINE TO IMPROVE ...RIBOSOMAL BINDING LOOP (WILD TYPE SEQUENCE REGION 46-67) REPLACED BY A SINGLE SERINE TO IMPROVE SOLUBILITY AND STABILITY
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
3D HNCO
1
3
1
3D HNCA
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3DC(CO)NH
1
7
1
3DHBHA(CO)NH
1
8
1
3D (H)CCH-TOCSY
1
9
1
3D CCH-TOCSY
1
10
1
3D 1H-15N NOESY
1
11
1
3D 1H-13C NOESY aliphatic
1
12
1
2D 1H-13C HSQC aliphatic
1
13
1
2D 1H-13C HSQC aromatic
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Sample preparation
Details
Contents: 0.9 mM [U-99% 13C; U-99% 15N] protein, 25 mM HEPES, 50 mM sodium chloride, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.9mM
entity-1
[U-99% 13C; U-99% 15N]
1
25mM
HEPES-2
1
50mM
sodium chloride-3
1
Sample conditions
pH: 7.5 / Pressure: ambient / Temperature: 323 K
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
800
1
Bruker Avance
Bruker
AVANCE
600
2
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Processing
NMR software
Name
Developer
Classification
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
structuresolution
NMRView
Johnson, OneMoonScientific
chemicalshiftassignment
NMRView
Johnson, OneMoonScientific
peakpicking
XPLOR
refinement
Refinement
Method: simulated annealing / Software ordinal: 1
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 160 / Conformers submitted total number: 20
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