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Yorodumi- PDB-2m5e: Structure of the C-domain of Calcium-saturated Calmodulin bound t... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2m5e | ||||||
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Title | Structure of the C-domain of Calcium-saturated Calmodulin bound to the IQ motif of NaV1.2 | ||||||
Components |
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Keywords | CALCIUM-BINDING PROTEIN/METAL TRANSPORT / Calcium Binding Protein / NaV1.2 / Ion Channel Gating / IQ Motif / Metal Binding / Sodium Channels / Metal Transport / Voltage Dependent / Voltage Gated / Calcium Binding Protein-Metal Transport Complex / Neuronal Peptides / EF-Hand / CALCIUM-BINDING PROTEIN-METAL TRANSPORT complex | ||||||
Function / homology | Function and homology information leucine zipper domain binding / sodium ion binding / axon initial segment / intrinsic apoptotic signaling pathway in response to osmotic stress / nerve development / corpus callosum development / paranode region of axon / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / dentate gyrus development / node of Ranvier ...leucine zipper domain binding / sodium ion binding / axon initial segment / intrinsic apoptotic signaling pathway in response to osmotic stress / nerve development / corpus callosum development / paranode region of axon / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / dentate gyrus development / node of Ranvier / voltage-gated sodium channel complex / membrane depolarization during action potential / voltage-gated sodium channel activity / optic nerve development / sodium ion transport / axon development / intercalated disc / sodium ion transmembrane transport / neuronal action potential / enzyme regulator activity / T-tubule / myelination / determination of adult lifespan / cerebral cortex development / memory / presynaptic membrane / nervous system development / cellular response to hypoxia / neuron apoptotic process / calmodulin binding / axon / glutamatergic synapse / calcium ion binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Paramecium tetraurelia (eukaryote) Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | minimized average structure, model 1 | ||||||
Model type details | minimized average | ||||||
Authors | Fowler, C.A. / Feldkamp, M.D. / Yu, L. / Shea, M.A. | ||||||
Citation | Journal: Biophys. Chem. / Year: 2017 Title: Calcium triggers reversal of calmodulin on nested anti-parallel sites in the IQ motif of the neuronal voltage-dependent sodium channel NaV1.2. Authors: Hovey, L. / Fowler, C.A. / Mahling, R. / Lin, Z. / Miller, M.S. / Marx, D.C. / Yoder, J.B. / Kim, E.H. / Tefft, K.M. / Waite, B.C. / Feldkamp, M.D. / Yu, L. / Shea, M.A. #1: Journal: Structure / Year: 2011 Title: Structural and Energetic Determinants of Apo Calmodulin Binding to the IQ Motif of the NaV1.2 Voltage-Dependent Sodium Channel Authors: Feldkamp, M.D. / Yu, L. / Shea, M.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m5e.cif.gz | 675.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2m5e.ent.gz | 563.8 KB | Display | PDB format |
PDBx/mmJSON format | 2m5e.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2m5e_validation.pdf.gz | 488.9 KB | Display | wwPDB validaton report |
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Full document | 2m5e_full_validation.pdf.gz | 745 KB | Display | |
Data in XML | 2m5e_validation.xml.gz | 35.6 KB | Display | |
Data in CIF | 2m5e_validation.cif.gz | 57.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m5/2m5e ftp://data.pdbj.org/pub/pdb/validation_reports/m5/2m5e | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8416.313 Da / Num. of mol.: 1 / Fragment: UNP residues 77-149 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Paramecium tetraurelia (eukaryote) / Strain: d4-2 / Gene: CAM, GSPATT00015825001 / Plasmid: PT7-7 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P07463 |
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#2: Protein/peptide | Mass: 3370.088 Da / Num. of mol.: 1 / Fragment: UNP residues 1901-1927 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Scn2a, Scn2a1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P04775 |
#3: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
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Sample |
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Sample conditions | pH: 6.8 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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