手法: 溶液NMR / 詳細: NMR structure refined with RDC data
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3D 1H-13C arom NOESY
1
7
1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
1
8
1
3DC(CO)NH
1
9
1
3D (H)CCH-TOCSY
1
10
2
2D 1H-13C HSQC
1
11
3
2D 1H-15N HSQC
-
試料調製
詳細
Solution-ID
内容
溶媒系
1
1.073 mM [U-100% 13C; U-100% 15N] OR16.004, 1 x Proteinase Inhibitors, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 200 mM NaCL, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.867 mM [U-100% 13C; U-100% 15N] OR16.006, 1 x Proteinase Inhibitors, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 200 mM NaCL, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.859 mM [U-100% 13C; U-100% 15N] OR16.013, 1 x Proteinase Inhibitors, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 200 mM NaCL, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O