Mass: 21374.805 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Micromonospora echinospora (bacteria) / Gene: calU16 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pMgK / References: UniProt: Q8KNE9
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3D 1H-13C NOESY aliphatic
1
7
1
3D 1H-13C NOESY aromatic
1
8
1
3D (H)CCH-TOCSY
1
9
2
3D (H)CCH-TOCSY
1
10
4
2D 1H-13C HSQC
1
11
2
4D CC-NOESY
1
12
1
3DHBHA(CO)NH
1
13
1
3D (H)CCH-COSY
1
14
1
3DC(CO)NH
1
15
1
3DH(CCO)NH
1
16
3
NUS 3D 1H-13C NOESY aliphatic
1
17
3
NUS 3D 1H-15N NOESY
1
18
1
3D 1H-15N NOESY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.9 mM [U-100% 13C; U-100% 15N] mir12.006, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.9 mM [U-100% 13C; U-100% 15N] mir12.006, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 100 % D2O, 50 uM DSS, 100% D2O
100% D2O
3
0.9 mM [U-100% 13C; U-100% 15N] mir12.011, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
4
0.9 mM [U-100% 15N], 5% 13C stereo mir12.008, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O