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基本情報
登録情報 | データベース: PDB / ID: 2lt9 | ||||||
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タイトル | The solution structure of Ca2+ binding domain 2B of the third isoform of the Na+/Ca2+ exchanger | ||||||
![]() | Protein Slc8a3 | ||||||
![]() | METAL BINDING PROTEIN / NCX / NCX3 / NaCa exchanger / CBD / CBD2 / calcium binding domain 2 | ||||||
機能・相同性 | ![]() calcium:monoatomic cation antiporter activity / monoatomic ion antiporter activity involved in regulation of postsynaptic membrane potential / calcium ion export across plasma membrane / calcium:monoatomic cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion concentration / : / Reduction of cytosolic Ca++ levels / Sodium/Calcium exchangers / : / regulation of skeletal muscle contraction / mitochondrial calcium ion transmembrane transport ...calcium:monoatomic cation antiporter activity / monoatomic ion antiporter activity involved in regulation of postsynaptic membrane potential / calcium ion export across plasma membrane / calcium:monoatomic cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion concentration / : / Reduction of cytosolic Ca++ levels / Sodium/Calcium exchangers / : / regulation of skeletal muscle contraction / mitochondrial calcium ion transmembrane transport / mitochondrial calcium ion homeostasis / calcium:sodium antiporter activity / metal ion transport / telencephalon development / cell communication / Ion homeostasis / negative regulation of protein serine/threonine kinase activity / calcium ion transport into cytosol / sodium ion transport / oligodendrocyte differentiation / plasma membrane => GO:0005886 / modulation of excitatory postsynaptic potential / sarcoplasm / calcium ion import across plasma membrane / sodium ion transmembrane transport / hematopoietic progenitor cell differentiation / axon terminus / cellular response to cAMP / myelination / learning / cell projection / calcium ion transmembrane transport / long-term synaptic potentiation / synapse organization / neuromuscular junction / sarcolemma / memory / intracellular calcium ion homeostasis / cellular response to hypoxia / perikaryon / microtubule / mitochondrial outer membrane / dendritic spine / postsynaptic density / learning or memory / calmodulin binding / axon / neuronal cell body / synapse / dendrite / endoplasmic reticulum membrane / perinuclear region of cytoplasm / mitochondrion / metal ion binding / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
![]() | Breukels, V. / Touw, W.G. / Vuister, G.W. | ||||||
![]() | ![]() タイトル: NMR structure note: solution structure of Ca(2+) binding domain 2B of the third isoform of the Na(+)/Ca (2+) exchanger. 著者: Breukels, V. / Touw, W.G. / Vuister, G.W. | ||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 1 MB | 表示 | ![]() |
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PDB形式 | ![]() | 897.5 KB | 表示 | ![]() |
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-検証レポート
文書・要旨 | ![]() | 415.1 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 568 KB | 表示 | |
XML形式データ | ![]() | 61.5 KB | 表示 | |
CIF形式データ | ![]() | 79.6 KB | 表示 | |
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-関連構造データ
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登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 17900.031 Da / 分子数: 1 / 断片: calcium binding domain 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 溶液NMR 詳細: The solution structure of calcium binding domain 2B of NCX3 is solved in excess of 10 mM CaCl2. However, Ca ions are not modeled in the structure, because resonances of crucial coordinating ...詳細: The solution structure of calcium binding domain 2B of NCX3 is solved in excess of 10 mM CaCl2. However, Ca ions are not modeled in the structure, because resonances of crucial coordinating residues are lacking: res 605 to 610 and 665 to 669. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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試料調製
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試料状態 | イオン強度: 20 / pH: 7.0 / 圧: ambient / 温度: 306 K |
-NMR測定
NMRスペクトロメーター |
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解析
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: THE SOLUTION STRUCTURE OF CALCIUM BINDING DOMAIN 2B OF NCX3 IS SOLVED IN EXCESS OF 10 MM CACL2. HOWEVER, CA IONS ARE NOT MODELED IN THE STRUCTURE, BECAUSE RESONANCES OF CRUCIAL COORDINATING ...詳細: THE SOLUTION STRUCTURE OF CALCIUM BINDING DOMAIN 2B OF NCX3 IS SOLVED IN EXCESS OF 10 MM CACL2. HOWEVER, CA IONS ARE NOT MODELED IN THE STRUCTURE, BECAUSE RESONANCES OF CRUCIAL COORDINATING RESIDUES ARE LACKING: RES 605 TO 610 AND 665 TO 669. THE REFINEMENT PROTOCOL IN EXPLICIT WATER, INCLUDING DISTANCE AND DIHEDRAL RESTRAINTS. THE RDC RESTRAINTS WERE NOT INCLUDED IN THE REFINEMENT. | ||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 2901 / NOE long range total count: 1289 / NOE medium range total count: 279 / Protein phi angle constraints total count: 93 / Protein psi angle constraints total count: 93 | ||||||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |