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Open data
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Basic information
Entry | Database: PDB / ID: 4ug3 | ||||||
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Title | B. subtilis GpsB N-terminal Domain | ||||||
![]() | CELL CYCLE PROTEIN GPSB | ||||||
![]() | CELL CYCLE / BACTERIAL GROWTH REGULATION / CELL WALL SYNTHESIS / CELL DIVISION | ||||||
Function / homology | Cell cycle protein GpsB / DivIVA family / DivIVA domain / DivIVA protein / regulation of cell shape / cell cycle / cell division / cytoplasm / Cell cycle protein GpsB![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rismondo, J. / Cleverley, R.M. / Lane, H.V. / Grohennig, S. / Steglich, A. / Moller, L. / Krishna Mannala, G. / Hain, T. / Lewis, R.J. / Halbedel, S. | ||||||
![]() | ![]() Title: Structure of the Bacterial Cell Division Determinant Gpsb and its Interaction with Penicillin Binding Proteins. Authors: Rismondo, J. / Cleverley, R.M. / Lane, H.V. / Grosshennig, S. / Steglich, A. / Moller, L. / Mannala, G.K. / Hain, T. / Lewis, R.J. / Halbedel, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58.8 KB | Display | ![]() |
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PDB format | ![]() | 44.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.2 KB | Display | ![]() |
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Full document | ![]() | 442.4 KB | Display | |
Data in XML | ![]() | 9.3 KB | Display | |
Data in CIF | ![]() | 12.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ug1SC ![]() 5an5C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 8413.601 Da / Num. of mol.: 4 / Fragment: N-TERMINAL DOMAIN, UNP RESIDUES 1-68 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.72 % / Description: NONE |
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Crystal grow | pH: 6.5 Details: 0.05M BIS TRIS PH 6.5, 0.05M AMMONIUM SULPHATE, 30% PENTAERYTHRITOL ETHOXYLATE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 14, 2015 |
Radiation | Monochromator: SI (111) DOUBLE CRYSTAL MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50.3 Å / Num. obs: 7791 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 3.7 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 10.6 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.49 / Mean I/σ(I) obs: 3.7 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4UG1 Resolution: 2.8→50.3 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.848 / SU B: 17.384 / SU ML: 0.338 / Cross valid method: THROUGHOUT / ESU R Free: 0.462 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 53.503 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→50.3 Å
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Refine LS restraints |
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