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- PDB-2lob: PDZ Domain of CAL (Cystic Fibrosis Transmembrane Regulator-Associ... -
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Basic information
Entry | Database: PDB / ID: 2lob | ||||||
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Title | PDZ Domain of CAL (Cystic Fibrosis Transmembrane Regulator-Associated Ligand) | ||||||
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![]() | PEPTIDE BINDING PROTEIN / STRUCTURAL PROTEIN-HYDROLASE complex | ||||||
Function / homology | ![]() negative regulation of anion channel activity / positive regulation of voltage-gated chloride channel activity / positive regulation of cyclic nucleotide-gated ion channel activity / Sec61 translocon complex binding / channel-conductance-controlling ATPase / intracellularly ATP-gated chloride channel activity / positive regulation of enamel mineralization / transepithelial water transport / RHO GTPases regulate CFTR trafficking / intracellular pH elevation ...negative regulation of anion channel activity / positive regulation of voltage-gated chloride channel activity / positive regulation of cyclic nucleotide-gated ion channel activity / Sec61 translocon complex binding / channel-conductance-controlling ATPase / intracellularly ATP-gated chloride channel activity / positive regulation of enamel mineralization / transepithelial water transport / RHO GTPases regulate CFTR trafficking / intracellular pH elevation / negative regulation of protein localization to cell surface / amelogenesis / chloride channel inhibitor activity / ATPase-coupled inorganic anion transmembrane transporter activity / Golgi-associated vesicle membrane / Golgi to plasma membrane transport / apical protein localization / multicellular organismal-level water homeostasis / molecular sequestering activity / trans-Golgi network transport vesicle / membrane hyperpolarization / cholesterol transport / bicarbonate transport / bicarbonate transmembrane transporter activity / vesicle docking involved in exocytosis / chloride channel regulator activity / chloride transmembrane transporter activity / sperm capacitation / chloride channel activity / RHOQ GTPase cycle / cholesterol biosynthetic process / positive regulation of exocytosis / positive regulation of insulin secretion involved in cellular response to glucose stimulus / chloride channel complex / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / endoplasmic reticulum to Golgi vesicle-mediated transport / cellular response to cAMP / cellular response to forskolin / chloride transmembrane transport / isomerase activity / response to endoplasmic reticulum stress / establishment of localization in cell / PDZ domain binding / Defective CFTR causes cystic fibrosis / Late endosomal microautophagy / clathrin-coated endocytic vesicle membrane / ABC-family proteins mediated transport / recycling endosome / transmembrane transport / Aggrephagy / Chaperone Mediated Autophagy / recycling endosome membrane / protein transport / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / early endosome membrane / protein-folding chaperone binding / transmembrane transporter binding / postsynaptic density / early endosome / endosome membrane / Ub-specific processing proteases / apical plasma membrane / lysosomal membrane / Golgi membrane / dendrite / endoplasmic reticulum membrane / Golgi apparatus / enzyme binding / cell surface / ATP hydrolysis activity / protein-containing complex / ATP binding / identical protein binding / membrane / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Piserchio, A. / Fellows, A. / Madden, D.R. / Mierke, D.F. | ||||||
![]() | ![]() Title: Association of the cystic fibrosis transmembrane regulator with CAL: structural features and molecular dynamics. Authors: Piserchio, A. / Fellows, A. / Madden, D.R. / Mierke, D.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 209 KB | Display | ![]() |
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PDB format | ![]() | 169 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 419.8 KB | Display | ![]() |
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Full document | ![]() | 552 KB | Display | |
Data in XML | ![]() | 29.1 KB | Display | |
Data in CIF | ![]() | 39.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 12528.140 Da / Num. of mol.: 1 / Fragment: PDZ domain residues 286-370 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 990.046 Da / Num. of mol.: 1 / Fragment: PDZ-binding motif residues 1473-1480 Source method: isolated from a genetically manipulated source Source: (synth.) ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1.0 mM [U-100% 13C; U-100% 15N] protein_1, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.0 mM / Component: entity_1-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 1.0 / pH: 6.8 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 7 |