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- PDB-2lfs: Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodime... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2lfs | ||||||
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Title | Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer; A219F variant | ||||||
![]() | HAMP domain-containing protein, Osmolarity sensor protein EnzV chimera | ||||||
![]() | TRANSFERASE / transmembrane signaling / HAMP domain / histidine kinase / gearbox model | ||||||
Function / homology | ![]() histidine kinase / phosphorelay sensor kinase activity / signal transduction / ATP binding / identical protein binding / membrane / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model type details | minimized average | ||||||
![]() | Coles, M. / Ferris, H.U. / Hulko, M. / Martin, J. / Lupas, A.N. | ||||||
![]() | ![]() Title: Mechanism of regulation of receptor histidine kinases. Authors: Ferris, H.U. / Dunin-Horkawicz, S. / Hornig, N. / Hulko, M. / Martin, J. / Schultz, J.E. / Zeth, K. / Lupas, A.N. / Coles, M. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.3 MB | Display | ![]() |
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PDB format | ![]() | 1.1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 410 KB | Display | ![]() |
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Full document | ![]() | 588.2 KB | Display | |
Data in XML | ![]() | 65.2 KB | Display | |
Data in CIF | ![]() | 98.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2lfrC ![]() 3zrvC ![]() 3zrwC ![]() 3zrxC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 13003.773 Da / Num. of mol.: 2 / Mutation: A291F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126 Gene: AF_1503, envZ, SF3423, S4340 / Production host: ![]() ![]() References: UniProt: O28769, UniProt: P0AEJ5, histidine kinase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: 0.150 / pH: 7.1 / Pressure: AMBIENT / Temperature: 308 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: NON-BONDED POTENTIAL SUPPLEMENTED WITH CONFORMATIONAL DATABASE POTENTIAL | ||||||||||||||||||||||||
NMR constraints | Protein chi angle constraints total count: 156 / Protein other angle constraints total count: 68 / Protein phi angle constraints total count: 224 / Protein psi angle constraints total count: 228 | ||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 19 | ||||||||||||||||||||||||
NMR ensemble rms | Distance rms dev: 0.014 Å / Distance rms dev error: 0.001 Å |