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Yorodumi- PDB-2lef: LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lef | |||||||||
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Title | LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES | |||||||||
Components |
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Keywords | GENE REGULATION/DNA / LEF1 / HMG / TCR-A / TRANSCRIPTION FACTOR / DNA BINDING / DNA BENDING / COMPLEX (HMG DOMAIN-DNA) / GENE REGULATION-DNA COMPLEX | |||||||||
Function / homology | Function and homology information Repression of WNT target genes / forebrain neuroblast division / trachea gland development / paraxial mesoderm formation / chorio-allantoic fusion / anatomical structure regression / RUNX3 regulates WNT signaling / negative regulation of interleukin-4 production / Regulation of MITF-M-dependent genes involved in cell cycle and proliferation / positive regulation of granulocyte differentiation ...Repression of WNT target genes / forebrain neuroblast division / trachea gland development / paraxial mesoderm formation / chorio-allantoic fusion / anatomical structure regression / RUNX3 regulates WNT signaling / negative regulation of interleukin-4 production / Regulation of MITF-M-dependent genes involved in cell cycle and proliferation / positive regulation of granulocyte differentiation / T cell receptor V(D)J recombination / negative regulation of interleukin-5 production / negative regulation of interleukin-13 production / neutrophil differentiation / negative regulation of apoptotic process in bone marrow cell / negative regulation of striated muscle tissue development / T-helper 1 cell differentiation / Formation of the beta-catenin:TCF transactivating complex / armadillo repeat domain binding / Deactivation of the beta-catenin transactivating complex / apoptotic process involved in blood vessel morphogenesis / formation of radial glial scaffolds / positive regulation of cell proliferation in bone marrow / Ca2+ pathway / apoptotic process involved in morphogenesis / odontoblast differentiation / secondary palate development / cell development / beta-catenin-TCF complex / gamma-catenin binding / positive regulation of gamma-delta T cell differentiation / positive regulation of odontoblast differentiation / mammary gland development / forebrain radial glial cell differentiation / positive regulation of chondrocyte proliferation / dentate gyrus development / vasculature development / C2H2 zinc finger domain binding / regulation of Wnt signaling pathway / positive regulation by host of viral transcription / transcription regulator inhibitor activity / embryonic limb morphogenesis / face morphogenesis / sprouting angiogenesis / tongue development / DNA binding, bending / alpha-beta T cell differentiation / epithelial cell apoptotic process / branching involved in blood vessel morphogenesis / odontogenesis of dentin-containing tooth / B cell proliferation / regulation of cell-cell adhesion / positive regulation of Wnt signaling pathway / regulation of neurogenesis / epithelial to mesenchymal transition / somitogenesis / canonical Wnt signaling pathway / cellular response to interleukin-4 / BMP signaling pathway / cis-regulatory region sequence-specific DNA binding / positive regulation of epithelial to mesenchymal transition / protein localization to chromatin / cell chemotaxis / transcription corepressor binding / hippocampus development / nuclear estrogen receptor binding / positive regulation of cell differentiation / DNA-binding transcription repressor activity, RNA polymerase II-specific / beta-catenin binding / histone deacetylase binding / osteoblast differentiation / RNA polymerase II transcription regulator complex / sensory perception of taste / histone binding / DNA-binding transcription activator activity, RNA polymerase II-specific / transcription regulator complex / sequence-specific DNA binding / transcription by RNA polymerase II / nucleic acid binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / positive regulation of cell migration / DNA-binding transcription factor activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription / positive regulation of gene expression / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | SOLUTION NMR / DG IN TORSION SPACE (DIANA), DYNAMICAL SIMULATED ANNEALING | |||||||||
Authors | Li, X. / Love, J.J. / Case, D.A. / Wright, P.E. | |||||||||
Citation | Journal: Nature / Year: 1995 Title: Structural basis for DNA bending by the architectural transcription factor LEF-1. Authors: Love, J.J. / Li, X. / Case, D.A. / Giese, K. / Grosschedl, R. / Wright, P.E. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lef.cif.gz | 570 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lef.ent.gz | 470 KB | Display | PDB format |
PDBx/mmJSON format | 2lef.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2lef_validation.pdf.gz | 381.7 KB | Display | wwPDB validaton report |
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Full document | 2lef_full_validation.pdf.gz | 577.7 KB | Display | |
Data in XML | 2lef_validation.xml.gz | 20.7 KB | Display | |
Data in CIF | 2lef_validation.cif.gz | 38.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/le/2lef ftp://data.pdbj.org/pub/pdb/validation_reports/le/2lef | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 4504.936 Da / Num. of mol.: 1 / Fragment: LEF-1 BINDING SITE / Source method: obtained synthetically |
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#2: DNA chain | Mass: 4674.046 Da / Num. of mol.: 1 / Fragment: LEF-1 BINDING SITE / Source method: obtained synthetically |
#3: Protein | Mass: 10403.220 Da / Num. of mol.: 1 / Fragment: HMG / Mutation: CHAIN A, C25S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Strain: BL21 (DE3) / Cell: PRE-B AND T LYMPHOCYTE / Gene: LEF1 / Plasmid: PET-21A / Species (production host): Escherichia coli / Gene (production host): LEF1 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P27782 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: PRESSURE: 1 ATM SOLVENT SYSTEM: 90%H2O/10%D2O, |
-Sample preparation
Sample conditions | Ionic strength: 10 mM KCL / pH: 6.7 / Pressure: 1 atm / Temperature: 303 K |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DG IN TORSION SPACE (DIANA), DYNAMICAL SIMULATED ANNEALING Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | |||||||||||||||
NMR ensemble | Conformer selection criteria: CONSTRAINTS VIOLATION, AMBER ENERGIES Conformers calculated total number: 28 / Conformers submitted total number: 12 |