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Yorodumi- PDB-2lbp: STRUCTURE OF THE L-LEUCINE-BINDING PROTEIN REFINED AT 2.4 ANGSTRO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lbp | ||||||
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Title | STRUCTURE OF THE L-LEUCINE-BINDING PROTEIN REFINED AT 2.4 ANGSTROMS RESOLUTION AND COMPARISON WITH THE LEU(SLASH)ILE(SLASH)VAL-BINDING PROTEIN STRUCTURE | ||||||
Components | LEUCINE-BINDING PROTEIN | ||||||
Keywords | PERIPLASMIC BINDING PROTEIN | ||||||
Function / homology | Function and homology information branched-chain amino acid transport / L-leucine binding / phenylalanine transport / L-leucine transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | ||||||
Authors | Sack, J.S. / Trakhanov, S.D. / Tsigannik, I.H. / Quiocho, F.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1989 Title: Structure of the L-leucine-binding protein refined at 2.4 A resolution and comparison with the Leu/Ile/Val-binding protein structure. Authors: Sack, J.S. / Trakhanov, S.D. / Tsigannik, I.H. / Quiocho, F.A. #1: Journal: J.Mol.Biol. / Year: 1989 Title: Periplasmic Binding Protein Structure and Function. Refined X-Ray Structures of the Leucine(Slash)Isoleucine(Slash)Valine-Binding Protein and its Complex with Leucine Authors: Sack, J.S. / Saper, M.A. / Quiocho, F.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lbp.cif.gz | 76.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lbp.ent.gz | 57.6 KB | Display | PDB format |
PDBx/mmJSON format | 2lbp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2lbp_validation.pdf.gz | 370.3 KB | Display | wwPDB validaton report |
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Full document | 2lbp_full_validation.pdf.gz | 409.5 KB | Display | |
Data in XML | 2lbp_validation.xml.gz | 13.7 KB | Display | |
Data in CIF | 2lbp_validation.cif.gz | 19.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lb/2lbp ftp://data.pdbj.org/pub/pdb/validation_reports/lb/2lbp | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 37006.664 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain (production host): K12 / References: UniProt: P04816 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.6 % | |||||||||||||||
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Crystal grow | *PLUS pH: 4.3 / Method: microdialysis | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 20 Å / Num. obs: 13797 / % possible obs: 95.6 % / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.1057 |
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-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.4→10 Å /
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Refinement step | Cycle: LAST / Resolution: 2.4→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 10 Å / Num. reflection obs: 13797 / Rfactor obs: 0.213 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_mcbond_it / Dev ideal: 0.662 |