BaculoviralIAPrepeat-containingprotein2 / C-IAP1 / IAP homolog B / Inhibitor of apoptosis protein 2 / IAP-2 / hIAP-2 / hIAP2 / RING finger ...C-IAP1 / IAP homolog B / Inhibitor of apoptosis protein 2 / IAP-2 / hIAP-2 / hIAP2 / RING finger protein 48 / TNFR2-TRAF-signaling complex protein 2
Mass: 15147.399 Da / Num. of mol.: 1 / Fragment: cIAP1-CARD (UNP RESIDUES 435-562) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: API1, BIRC2, IAP2, MIHB, RNF48 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q13490
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR / Details: Caspase recruitment domain of cIAP1
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-1H NOESY
1
2
2
2D 1H-15N HSQC
1
3
3
3D HNCA
1
4
3
3D HN(CA)CB
1
5
3
CC(CO)NH
1
6
3
3D HNCO
1
7
3
3D (H)CCH-TOCSY
1
8
2
3D HNHA
1
9
2
3D HNHB
1
10
2
3D 1H-15N NOESY
1
11
3
3D 1H-13C NOESY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1 mM cIAP1-CARD-1, 20 mM sodium phosphate-2, 50 mM sodium chloride-3, 5 mM TCEP-4, 90% H2O/10% D2O
90% H2O/10% D2O
2
1 mM [U-100% 15N] cIAP1-CARD-5, 20 mM sodium phosphate-6, 50 mM sodium chloride-7, 5 mM TCEP-8, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.4 mM [U-100% 13C; U-100% 15N] cIAP1-CARD-9, 20 mM sodium phosphate-10, 50 mM sodium chloride-11, 5 mM TCEP-12, 90% H2O/10% D2O
NOE constraints total: 2783 / NOE intraresidue total count: 799 / NOE long range total count: 591 / NOE medium range total count: 744 / NOE sequential total count: 649
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 256 / Conformers submitted total number: 20 / Representative conformer: 1
NMR ensemble rms
Distance rms dev: 0.015 Å / Distance rms dev error: 0.002 Å
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