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Yorodumi- PDB-2l4s: Promiscuous Binding at the Crossroads of Numerous Cancer Pathways... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2l4s | ||||||
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Title | Promiscuous Binding at the Crossroads of Numerous Cancer Pathways: Insight from the Binding of GIP with Glutaminase L | ||||||
Components | Tax1-binding protein 3 | ||||||
Keywords | PEPTIDE BINDING PROTEIN / PDZ domain / GIP / Glutatminase L | ||||||
Function / homology | Function and homology information negative regulation of protein localization to cell surface / RHO GTPases Activate Rhotekin and Rhophilins / activation of GTPase activity / negative regulation of Wnt signaling pathway / Rho protein signal transduction / fibrillar center / beta-catenin binding / Wnt signaling pathway / actin cytoskeleton / negative regulation of cell population proliferation ...negative regulation of protein localization to cell surface / RHO GTPases Activate Rhotekin and Rhophilins / activation of GTPase activity / negative regulation of Wnt signaling pathway / Rho protein signal transduction / fibrillar center / beta-catenin binding / Wnt signaling pathway / actin cytoskeleton / negative regulation of cell population proliferation / intracellular membrane-bounded organelle / extracellular exosome / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | ||||||
Authors | Zoetewey, D.L. / Ovee, M. / Banerjee, M. / Bhaskaran, R. / Mohanty, S. | ||||||
Citation | Journal: Biochemistry / Year: 2011 Title: Promiscuous binding at the crossroads of numerous cancer pathways: insight from the binding of glutaminase interacting protein with glutaminase L. Authors: Zoetewey, D.L. / Ovee, M. / Banerjee, M. / Bhaskaran, R. / Mohanty, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2l4s.cif.gz | 839.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2l4s.ent.gz | 715.6 KB | Display | PDB format |
PDBx/mmJSON format | 2l4s.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l4/2l4s ftp://data.pdbj.org/pub/pdb/validation_reports/l4/2l4s | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13751.685 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TAX1BP3, TIP1 / Plasmid: pET-3c/GIP / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): pLysS / References: UniProt: O14907 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5-1 mM [U-99% 13C; U-99% 15N] Glutaminase Interacting Protein 3, 50 mM sodium phosphate, 0.1% w/v sodium azide, 1 mM EDTA, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 50 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |