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- PDB-1poz: SOLUTION STRUCTURE OF THE HYALURONAN BINDING DOMAIN OF HUMAN CD44 -
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Open data
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Basic information
Entry | Database: PDB / ID: 1poz | |||||||||
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Title | SOLUTION STRUCTURE OF THE HYALURONAN BINDING DOMAIN OF HUMAN CD44 | |||||||||
![]() | CD44 antigen | |||||||||
![]() | CELL ADHESION / HYALURONAN-BINDING DOMAIN / CARBOHYDRATE-BINDING DOMAIN / LINK MODULE / GLYCOPROTEIN | |||||||||
Function / homology | ![]() positive regulation of monocyte aggregation / Hyaluronan uptake and degradation / hyaluronic acid binding / neutrophil degranulation / monocyte aggregation / macrophage migration inhibitory factor receptor complex / regulation of lamellipodium morphogenesis / hyaluronan catabolic process / cellular response to fibroblast growth factor stimulus / positive regulation of heterotypic cell-cell adhesion ...positive regulation of monocyte aggregation / Hyaluronan uptake and degradation / hyaluronic acid binding / neutrophil degranulation / monocyte aggregation / macrophage migration inhibitory factor receptor complex / regulation of lamellipodium morphogenesis / hyaluronan catabolic process / cellular response to fibroblast growth factor stimulus / positive regulation of heterotypic cell-cell adhesion / cartilage development / wound healing, spreading of cells / cytokine receptor activity / leukocyte migration / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / plasma membrane => GO:0005886 / negative regulation of DNA damage response, signal transduction by p53 class mediator / microvillus / lamellipodium membrane / extracellular matrix disassembly / Integrin cell surface interactions / type II interferon-mediated signaling pathway / collagen binding / T cell activation / Degradation of the extracellular matrix / extracellular matrix organization / cell-matrix adhesion / secretory granule membrane / cell projection / Cell surface interactions at the vascular wall / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / cell-cell adhesion / transmembrane signaling receptor activity / positive regulation of peptidyl-tyrosine phosphorylation / Interferon gamma signaling / cell migration / positive regulation of peptidyl-serine phosphorylation / basolateral plasma membrane / positive regulation of ERK1 and ERK2 cascade / cell adhesion / inflammatory response / apical plasma membrane / focal adhesion / Neutrophil degranulation / negative regulation of apoptotic process / Golgi apparatus / cell surface / extracellular exosome / plasma membrane / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | SOLUTION NMR / torsion angle dynamics simulated annealing refinement by molecular dynamics | |||||||||
![]() | Teriete, P. / Banerji, S. / Blundell, C.D. / Kahmann, J.D. / Pickford, A.R. / Wright, A.J. / Campbell, I.D. / Jackson, D.G. / Day, A.J. | |||||||||
![]() | ![]() Title: Structure of the Regulatory Hyaluronan Binding Domain in the Inflammatory Leukocyte Homing Receptor CD44. Authors: Teriete, P. / Banerji, S. / Noble, M. / Blundell, C.D. / Wright, A.J. / Pickford, A.R. / Lowe, E. / Mahoney, D.J. / Tammi, M.I. / Kahmann, J.D. / Campbell, I.D. / Day, A.J. / Jackson, D.G. #1: ![]() Title: A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family. Authors: Stamenkovic, I. / Amiot, M. / Pesando, J.M. / Seed, B. #2: ![]() Title: A human lymphocyte homing receptor, the hermes antigen, is related to cartilage proteoglycan core and link proteins. Authors: A Goldstien, L. / Zhou, D.F.H. / Picker, L.J. / Minty, C.N. / Bargatze, R.F. / Din, J.F. / Butcher, E.C. #3: ![]() Title: CD44 is the principal cell surface receptor for hyaluronate. Authors: Aruffo, A. / Stamenkovic, I. / Melnick, M. / Underhill, C.B. / Seed, B. #4: ![]() Title: Identification of hyaluronic acid binding sites in the extracellular domain of CD44. Authors: Peach, R.J. / Hollenbaugh, D. / Stamenkovic, I. / Aruffo, A. #5: ![]() Title: Solution Structure of the Link Module: A Hyaluronan-Binding Domain Involved in Extracellular Matrix Stability and Cell Migration Authors: Kohda, D. / Morton, C.J. / Parkar, A.A. / Hatanaka, H. / Inagaki, F.M. / Campbell, I.D. / Day, A.J. #6: ![]() Title: Identification of CD44 residues important for hyaluronan binding and delineation of the binding site Authors: Bajorath, J. / Greenfield, B. / Munro, S.B. / Day, A.J. / Aruffo, A. #7: ![]() Title: Site-specific de-N-glycosylation of CD44 can activate hyaluronan binding, and CD44 activation states show distinct threshold densities for hyaluronan binding Authors: English, N.M. / Lesley, J.F. / Hyman, R. #8: ![]() Title: Characterization of a functional hyaluronan-binding domain from the human CD44 molecule expressed in Escherichia coli Authors: Banerji, S. / Day, A.J. / Kahmann, J.D. / Jackson, D.G. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 935.6 KB | Display | ![]() |
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PDB format | ![]() | 804.3 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 346.9 KB | Display | ![]() |
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Full document | ![]() | 641.9 KB | Display | |
Data in XML | ![]() | 107 KB | Display | |
Data in CIF | ![]() | 140.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 17601.486 Da / Num. of mol.: 1 / Fragment: HYALURONAN BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: <5mM / pH: 6.5 / Pressure: ambient / Temperature: 298 K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics simulated annealing refinement by molecular dynamics Software ordinal: 1 Details: The structures are calculated from 2271 restraints, 2168 are NOE based, 47 dihedral constraints and 56 distance restraints from hydrogen bonds | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy,target function Conformers calculated total number: 500 / Conformers submitted total number: 20 |