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Yorodumi- PDB-2knh: The Solution structure of the eTAFH domain of AML1-ETO complexed ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2knh | ||||||
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Title | The Solution structure of the eTAFH domain of AML1-ETO complexed with HEB peptide | ||||||
Components |
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Keywords | TRANSCRIPTION REGULATOR / AML1-ETO / eTAFH / HEB / DNA-binding / Metal-binding / Nucleus / Proto-oncogene / Transcription / Transcription regulation / Zinc-finger / Developmental protein / Phosphoprotein | ||||||
Function / homology | Function and homology information response to gonadotropin-releasing hormone / cAMP response element binding / HMG box domain binding / bHLH transcription factor binding / NGF-stimulated transcription / muscle organ development / negative regulation of fat cell differentiation / Myogenesis / E-box binding / SMAD binding ...response to gonadotropin-releasing hormone / cAMP response element binding / HMG box domain binding / bHLH transcription factor binding / NGF-stimulated transcription / muscle organ development / negative regulation of fat cell differentiation / Myogenesis / E-box binding / SMAD binding / cis-regulatory region sequence-specific DNA binding / positive regulation of neuron differentiation / nuclear matrix / RNA polymerase II transcription regulator complex / transcription corepressor activity / sequence-specific double-stranded DNA binding / nervous system development / RUNX1 regulates transcription of genes involved in differentiation of HSCs / gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / nuclear speck / immune response / protein heterodimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / DNA-templated transcription / positive regulation of gene expression / chromatin / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Park, S. / Cierpicki, T. / Tonelli, M. / Bushweller, J.H. | ||||||
Citation | Journal: Blood / Year: 2009 Title: Structure of the AML1-ETO eTAFH domain-HEB peptide complex and its contribution to AML1-ETO activity. Authors: Park, S. / Chen, W. / Cierpicki, T. / Tonelli, M. / Cai, X. / Speck, N.A. / Bushweller, J.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2knh.cif.gz | 751.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2knh.ent.gz | 629 KB | Display | PDB format |
PDBx/mmJSON format | 2knh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kn/2knh ftp://data.pdbj.org/pub/pdb/validation_reports/kn/2knh | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11623.448 Da / Num. of mol.: 1 / Fragment: eTAFH domain (UNP residues 119-216) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RUNX1T1, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q06455 |
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#2: Protein/peptide | Mass: 1990.214 Da / Num. of mol.: 1 / Fragment: HEB peptide (UNP residues 11-28) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: Q99081 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 25 mM Bis-Tris, 1 mM EDTA, 350 mM sodium chloride, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 350 / pH: 6 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |