- PDB-2kcz: Solution NMR structure of the C-terminal domain of protein DR_A00... -
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Basic information
Entry
Database: PDB / ID: 2kcz
Title
Solution NMR structure of the C-terminal domain of protein DR_A0006 from Deinococcus radiodurans. Northeast Structural Genomics Consortium Target DrR147D
Components
Uncharacterized protein DR_A0006
Keywords
STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Protein structure / NESG / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium
Function / homology
: / Coenzyme Q-binding protein COQ10, START domain / Polyketide cyclase / dehydrase and lipid transport / START domain / Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4 / START-like domain superfamily / 2-Layer Sandwich / Alpha Beta / Coenzyme Q-binding protein COQ10 START domain-containing protein
Mass: 17378.764 Da / Num. of mol.: 1 / Fragment: UNP residues 98-243 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Deinococcus radiodurans R1 (radioresistant) Strain: R1 / DSM 20539 / IFO 15346 / LMG 4051 / NCIB 9279 / Gene: DR_A0006 / Plasmid: pET21-23C / Production host: Escherichia coli (E. coli) / References: UniProt: Q9RZE3
Sequence details
AUTHORS STATE THAT ALA AT POSITION 59 COMPARED TO THR IN THE SEQUENCE REFERENCE IS NOT A MUTATION ...AUTHORS STATE THAT ALA AT POSITION 59 COMPARED TO THR IN THE SEQUENCE REFERENCE IS NOT A MUTATION BUT NATURALLY OCCURING STRAIN VARIATION.
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3D HN(CA)CB
1
5
1
3D HNCA
1
6
1
3DCBCA(CO)NH
1
7
1
3DHBHA(CO)NH
1
8
1
3D (H)CCH-COSY
1
9
1
3D simultaneous NCaliCaro HH NOESY
1
10
2
2D 1H-13C HSQC
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.9 mM [U-5% 13C; U-99% 15N] DrR147D-10, 10 % [U-2H] D2O-11, 90 % H2O-12, 50 uM DSS-13, 10 mM DTT-14, 200 mM sodium chloride-15, 0.02 % sodium azide-16, 5 mM calcium chloride-17, 20 mM ammonium acetate-18, 90% H2O/10% D2O
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