Sodiumchannelproteintype2subunitalpha / Sodium channel protein type II subunit alpha / Voltage-gated sodium channel subunit alpha Nav1.2 / ...Sodium channel protein type II subunit alpha / Voltage-gated sodium channel subunit alpha Nav1.2 / Sodium channel protein / brain II subunit alpha / HBSC II
Mass: 14591.391 Da / Num. of mol.: 1 / Fragment: C-terminal EF-Hand Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Species: sapiens / Gene: NAC2, SCN2A, SCN2A1, SCN2A2 / Species (production host): coli / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q99250
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR Details: Solution structure of the human Voltage-gated Sodium Channel, brain isoform (Nav1.2)
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D HNCO
1
2
1
3D HNCA
1
3
1
3DHN(CO)CA
1
4
1
3DHN(CA)CO
1
5
1
3D HN(CA)CB
1
6
1
3DCBCA(CO)NH
1
7
1
3D (H)CCH-TOCSY
1
8
1
3DHBHA(CO)NH
1
9
1
3D 1H-15N NOESY
1
10
1
3D 1H-13C NOESY
1
11
2
2D 1H-13C HSQC
1
12
3
3D HCACO
1
13
3
2D IPAPHSQC
1
14
3
3D quant HNCO
1
15
3
3D CCa HNCO
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.5 mM [U-99% 13C; U-99% 15N] protein, 100 mM [D5-98%] glycine, 20 mM [D11-98 %] TRIS, 0.1 mM [D16-98%] EDTA, 10 % [U-99% 2H] D2O, 1 mM [D10-98%] DTT, 0.02 % NaN3, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.5 mM [U-10% 13C; U-99% 15N] protein, 100 mM [D5-98%] glycine, 20 mM [D11-98 %] TRIS, 0.1 mM [D16-98%] EDTA, 10 % [U-99% 2H] D2O, 1 mM [D10-98%] DTT, 0.02 % NaN3, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.5 mM [U-99% 13C; U-99% 15N] protein, 100 mM [D5-98%] glycine, 20 mM [D11-98 %] TRIS, 0.1 mM [D16-98%] EDTA, 10 % [U-99% 2H] D2O, 1 mM [D10-98%] DTT, 0.02 % NaN3, 15 mg pF1 phage, 90% H2O/10% D2O
NOE constraints total: 1772 / NOE intraresidue total count: 699 / NOE long range total count: 310 / NOE medium range total count: 321 / NOE sequential total count: 442 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 65 / Protein psi angle constraints total count: 65
NMR representative
Selection criteria: lowest energy
NMR ensemble
Average torsion angle constraint violation: 2.071 ° Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 15 / Maximum lower distance constraint violation: 0.25 Å / Maximum torsion angle constraint violation: 6.99 ° / Maximum upper distance constraint violation: 0.42 Å Torsion angle constraint violation method: ensemble average of rmsd
NMR ensemble rms
Distance rms dev: 0.021 Å / Distance rms dev error: 0.021 Å
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