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- PDB-2jxv: Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. e... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2jxv | ||||||
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Title | Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans | ||||||
![]() | RNA (33-MER) | ||||||
![]() | RNA / ASYMMETRIC INTERNAL LOOP / stem-loop / GAAA TETRALOOP / GU MISMATCH / RESIDUAL DIPOLAR COUPLINGS | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Method | SOLUTION NMR / simulated annealing, energy minimization | ||||||
![]() | Cevec, M. / Thibaudeau, C. / Plavec, J. | ||||||
![]() | ![]() Title: Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans. Authors: Cevec, M. / Thibaudeau, C. / Plavec, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 207.4 KB | Display | ![]() |
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PDB format | ![]() | 174 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 332.8 KB | Display | ![]() |
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Full document | ![]() | 382.3 KB | Display | |
Data in XML | ![]() | 4.2 KB | Display | |
Data in CIF | ![]() | 7.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 10625.362 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was prepared by in vitro transcription using T7 RNA polymerase and DNA oligonucleotide template. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing, energy minimization / Software ordinal: 1 Details: 693 NOE-derived distance restraints, 130 torsion angle restraints, 51 residual dipolar coupling restraints, 32 hydrogen bond restraints and 22 planarity restraints. | ||||||||||||||||
NMR constraints | NOE constraints total: 697 / NOE intraresidue total count: 370 / NOE medium range total count: 57 / NOE sequential total count: 270 | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy and least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 10 |