- PDB-2jxm: Ensemble of twenty structures of the Prochlorothrix hollandica pl... -
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基本情報
登録情報
データベース: PDB / ID: 2jxm
タイトル
Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex
要素
Cytochrome f
Plastocyanin
キーワード
ELECTRON TRANSPORT / Copper / Metal-binding / Transport
機能・相同性
機能・相同性情報
: / electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity / plasma membrane-derived thylakoid membrane / photosynthesis / electron transfer activity / iron ion binding / copper ion binding / heme binding 類似検索 - 分子機能
Plastocyanin, cyanobacteria / Cytochrome f large domain / Cytochrome f / Cytochrome f large domain / Cytochrome f large domain superfamily / Apocytochrome F, C-terminal / Apocytochrome F, N-terminal / Cytochrome f family profile. / Plastocyanin / Blue (type 1) copper protein, plastocyanin-type ...Plastocyanin, cyanobacteria / Cytochrome f large domain / Cytochrome f / Cytochrome f large domain / Cytochrome f large domain superfamily / Apocytochrome F, C-terminal / Apocytochrome F, N-terminal / Cytochrome f family profile. / Plastocyanin / Blue (type 1) copper protein, plastocyanin-type / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Blue (type 1) copper domain / Copper binding proteins, plastocyanin/azurin family / Blue (type 1) copper protein, binding site / Type-1 copper (blue) proteins signature. / Rudiment single hybrid motif / Cupredoxins - blue copper proteins / Cupredoxin / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Immunoglobulin-like / Beta Barrel / Sandwich / Mainly Beta 類似検索 - ドメイン・相同性
COPPER (II) ION / HEME C / Plastocyanin / Cytochrome f 類似検索 - 構成要素
内容: 85 uM [U-99% 15N] plastocyanin, 50 uM cytochrome f, 95% H2O/5% D2O 溶媒系: 95% H2O/5% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
85uM
plastocyanin
[U-99% 15N]
1
50uM
cytochromef
1
試料状態
イオン強度: 10 / pH: 6.0 / 圧: ambient / 温度: 300 K
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NMR測定
NMRスペクトロメーター
タイプ: Bruker DMX / 製造業者: Bruker / モデル: DMX / 磁場強度: 600 MHz
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解析
NMR software
名称
開発者
分類
TopSpin
BrukerBiospin
collection
Azara
Boucher, W.
解析
Azara
Boucher, W.
データ解析
ANSIG
Kraulis, P.J.
解析
ANSIG
Kraulis, P.J.
データ解析
X-PLOR NIH
Schwieters, C.D. etal.
精密化
精密化
手法: Rigid Body docking, Energy minimisation / ソフトェア番号: 1 詳細: Rigid Body docking based on PDB entry 1B3I and a homology model of cytochrome f. Docking energies based on pseudocontact and chemical shift perturbation restraints. Energy minimisation of side-chains.
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 1000 / 登録したコンフォーマーの数: 20