early viral transcription / late viral transcription / 7SK snRNA binding / cyclin/CDK positive transcription elongation factor complex / regulation of muscle cell differentiation / snRNA transcription by RNA polymerase II / cyclin-dependent protein serine/threonine kinase activator activity / positive regulation of DNA-templated transcription, elongation / RNA polymerase binding / cyclin-dependent protein serine/threonine kinase regulator activity ...early viral transcription / late viral transcription / 7SK snRNA binding / cyclin/CDK positive transcription elongation factor complex / regulation of muscle cell differentiation / snRNA transcription by RNA polymerase II / cyclin-dependent protein serine/threonine kinase activator activity / positive regulation of DNA-templated transcription, elongation / RNA polymerase binding / cyclin-dependent protein serine/threonine kinase regulator activity / regulation of cyclin-dependent protein serine/threonine kinase activity / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / RNA polymerase II transcribes snRNA genes / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / skeletal muscle tissue development / RNA Polymerase II Pre-transcription Events / transcription elongation by RNA polymerase II / TP53 Regulates Transcription of DNA Repair Genes / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / transcription coactivator binding / transcription by RNA polymerase II / cell cycle / cell division / chromatin binding / host cell nucleus / regulation of transcription by RNA polymerase II / protein kinase binding / perinuclear region of cytoplasm / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / plasma membrane / cytosol Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 327 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, GLN 130 TO ARG ENGINEERED RESIDUE IN CHAIN B, GLN 130 TO ARG ...ENGINEERED RESIDUE IN CHAIN A, GLN 130 TO ARG ENGINEERED RESIDUE IN CHAIN B, GLN 130 TO ARG REGULATORY SUBUNIT OF THE CYCLIN-DEPENDENT KINASE PAIR (CDK9/CYCLIN T) COMPLEX, ALSO CALLED POSITIVE TRANSCRIPTION ELONGATION FACTOR B (P-TEFB), WHICH IS PROPOSED TO FACILITATE THE TRANSITION FROM ABORTIVE TO PRODUCTION ELONGATION BY PHOSPHORYLATING THE CTD (CARBOXY-TERMINAL DOMAIN) OF THE LARGE SUBUNIT OF RNA POLYMERASE II (RNAP II).
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.79 Å3/Da / Density % sol: 55 %
Crystal grow
Method: vapor diffusion, sitting drop Details: 0.2M NA-FORMATE, 0.1M BIS-TRIS-PROPANE PH 8.5, 20%PEG3350, 10% ETHYLENE GLYCOL, 150 NL SITTING DROP