+
Open data
-
Basic information
Entry | Database: PDB / ID: 2iic | ||||||
---|---|---|---|---|---|---|---|
Title | Calcium bound structure of alpha-11 giardin | ||||||
![]() | Alpha-11 giardin | ||||||
![]() | METAL BINDING PROTEIN / calcium-protein complex / helix-turn-helix | ||||||
Function / homology | ![]() 1-phosphatidylinositol binding / phosphatidylethanolamine binding / calcium-dependent phospholipid binding / phosphatidylserine binding / cytoskeleton organization / heparin binding / microtubule / calcium ion binding / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pathuri, P. / Nguyen, E.T. / Luecke, H. | ||||||
![]() | ![]() Title: Apo and Calcium-bound Crystal Structures of Alpha-11 Giardin, an Unusual Annexin from Giardia lamblia Authors: Pathuri, P. / Nguyen, E.T. / Svard, S.G. / Luecke, H. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 246.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 198.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 458.4 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 506.2 KB | Display | |
Data in XML | ![]() | 48.8 KB | Display | |
Data in CIF | ![]() | 66.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
3 | ![]()
| ||||||||
4 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 35252.930 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.74 Å3/Da / Density % sol: 50.2 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 100mM Bis-Tris pH 6.5, 24% PEG 550 MME, 50mM calcium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 19, 2006 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Redundancy: 3.7 % / Av σ(I) over netI: 6.8 / Number: 112381 / Rmerge(I) obs: 0.104 / Χ2: 1.12 / D res high: 2.91 Å / D res low: 50 Å / Num. obs: 30731 / % possible obs: 99.6 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Diffraction reflection shell |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.91→50 Å / Num. obs: 30731 / % possible obs: 99.6 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.104 / Χ2: 1.117 / Net I/σ(I): 6.8 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Resolution: 2.91→3.01 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.493 / Num. unique all: 3054 / Χ2: 0.99 / % possible all: 99.4 |
-Phasing
Phasing MR |
|
---|
-
Processing
Software |
| ||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||||||||||||||
Solvent computation | Bsol: 29.473 Å2 | ||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.595 Å2 | ||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.93→40 Å
| ||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||
Xplor file |
|