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Yorodumi- PDB-2i3b: Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase -
+Open data
-Basic information
Entry | Database: PDB / ID: 2i3b | ||||||
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Title | Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase | ||||||
Components | Human Cancer-Related NTPase | ||||||
Keywords | HYDROLASE / NTPase / AAA / Rossmann | ||||||
Function / homology | Function and homology information : / ribonucleoside triphosphate phosphatase activity / nucleoside-triphosphate phosphatase / RNA binding / ATP binding / membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Placzek, W.J. / Almeida, M.S. / Wuthrich, K. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: NMR Structure and Functional Characterization of a Human Cancer-related Nucleoside Triphosphatase. Authors: Placzek, W.J. / Almeida, M.S. / Wuthrich, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2i3b.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2i3b.ent.gz | 988.2 KB | Display | PDB format |
PDBx/mmJSON format | 2i3b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i3/2i3b ftp://data.pdbj.org/pub/pdb/validation_reports/i3/2i3b | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 20610.869 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CDRT1 / Plasmid: pET21d / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: Q5TDE9, UniProt: Q9BSD7*PLUS, nucleoside-triphosphate phosphatase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2 mM HCR-NTPase U-15N,13C; 25 mM MES buffer pH 6.4; 10 mM MgCl2; 5 mM DTT; 2 mM ATPgS; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 25mM MES, 10mM MgCl2 / pH: 6.4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 80 / Conformers submitted total number: 20 |