Anti-SilencingFactor1paraloga / ASF1a protein / ASF1 anti-silencing function 1 homolog A / Hypothetical protein FLJ22085 / Anti- ...ASF1a protein / ASF1 anti-silencing function 1 homolog A / Hypothetical protein FLJ22085 / Anti-silencing function 1A / CGI-98 protein / CIA
Mass: 20837.066 Da / Num. of mol.: 2 / Fragment: Residues 1-157 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ASF1A, DKFZp564E2182 / Plasmid: modified pET_Duet (Novagen) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21_Gold (DE3) / References: UniProt: Q9Y294
#2: Protein
HistoneRegulatoryhomologA / HIRA protein / TUP1-like enhancer of split protein 1
Mass: 6215.963 Da / Num. of mol.: 2 / Fragment: Residues 425-472 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HIRA, DGCR1, HIR, TUPLE1 / Plasmid: modified pCDF_Duet vector (Novagen) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21_Gold(DE3) / References: UniProt: P54198
Mass: 18.015 Da / Num. of mol.: 130 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.02 Å3/Da / Density % sol: 59.25 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: CRYSTALS OF THE HUMAN ASF1AN-HIRA(425-472) COMPLEX WERE GROWN BY HANGING DROP VAPOR DIFFUSION AT ROOM TEMPERATURE AND WERE OBTAINED BY MIXING 2 UL OF A 0.5 MM PROTEIN COMPLEX SOLUTION (IN 20 ...Details: CRYSTALS OF THE HUMAN ASF1AN-HIRA(425-472) COMPLEX WERE GROWN BY HANGING DROP VAPOR DIFFUSION AT ROOM TEMPERATURE AND WERE OBTAINED BY MIXING 2 UL OF A 0.5 MM PROTEIN COMPLEX SOLUTION (IN 20 MM HEPES PH 7.0, 150 MM NACL AND 5 MM BETA-ME) WITH 2 UL OF RESERVOIR SOLUTION CONTAINING 1.44 M NAH2PO4 AND 0.16 M K2HPO4 AT PH 5.6, AND EQUILIBRATING OVER 1.0 ML OF RESERVOIR SOLUTION. CRYSTALS WERE FULLY GROWN WITHIN TWO WEEKS TO A TYPICAL SIZE OF 0.3MMX0.3MMX0.2MM, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298.0K
Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.98 Å / Relative weight: 1
Reflection
Av σ(I) over netI: 12.8 / Number: 200485 / Rmerge(I) obs: 0.058 / Χ2: 1.01 / D res high: 2.5 Å / D res low: 50 Å / Num. obs: 23857 / % possible obs: 99.9
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