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Open data
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Basic information
Entry | Database: PDB / ID: 2i21 | ||||||
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Title | Bacteriorhodopsin/lipid complex, T46V mutant | ||||||
![]() | Bacteriorhodopsin | ||||||
![]() | TRANSPORT PROTEIN / ION PUMP / MEMBRANE PROTEIN / RETINAL PROTEIN / LIPIDS / PHOTORECEPTOR / HALOARCHAEA / 7-TRANSMEMBRANE / SERPENTINE / ION TRANSPORT | ||||||
Function / homology | ![]() photoreceptor activity / phototransduction / proton transmembrane transport / monoatomic ion channel activity / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lanyi, J.K. / Schobert, B. | ||||||
![]() | ![]() Title: Propagating Structural Perturbation Inside Bacteriorhodopsin: Crystal Structures of the M State and the D96A and T46V Mutants. Authors: Lanyi, J.K. / Schobert, B. | ||||||
History |
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Remark 600 | SCHIFF BASE LINKAGE BETWEEN LYS 216 (NZ) AND RET 300 (C15) DIETHER LIPID BILAYER |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.8 KB | Display | ![]() |
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PDB format | ![]() | 49 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3 MB | Display | ![]() |
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Full document | ![]() | 3.1 MB | Display | |
Data in XML | ![]() | 17 KB | Display | |
Data in CIF | ![]() | 20.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2i1xC ![]() 2i20C ![]() 1c3wS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26927.525 Da / Num. of mol.: 1 / Mutation: T46V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() | ||||
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#2: Chemical | ChemComp-RET / | ||||
#3: Chemical | ChemComp-LI1 / #4: Chemical | ChemComp-SQU / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.82 Å3/Da / Density % sol: 43.97 % |
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Crystal grow | Temperature: 295 K / Method: cubic lipid phase / pH: 5.6 Details: MO:WATER:PHOSPHATE, pH 5.6, CUBIC LIPID PHASE, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM / Detector: CCD / Date: Apr 15, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97977 Å / Relative weight: 1 |
Reflection | Resolution: 1.84→25 Å / Num. all: 19393 / Num. obs: 19393 / % possible obs: 95.5 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.041 / Net I/σ(I): 28.9 |
Reflection shell | Resolution: 1.84→1.92 Å / Rmerge(I) obs: 0.537 / Mean I/σ(I) obs: 4.1 / % possible all: 90.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1C3W Resolution: 1.84→25 Å / Num. parameters: 8300 / Num. restraintsaints: 8223 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER / Details: NO MEROHEDRAL TWINNING
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Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 2074 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.84→25 Å
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Refine LS restraints |
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