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Yorodumi- PDB-2hn8: Structural characterization and oligomerization of PB1-F2, a pro-... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2hn8 | ||||||
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Title | Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein | ||||||
Components | Protein PB1-F2 | ||||||
Keywords | VIRAL PROTEIN / pro-apoptotic mitochondrial targeting protein | ||||||
Function / homology | Function and homology information host cell mitochondrial inner membrane / Influenza Virus Induced Apoptosis / host cell cytosol / Viral mRNA Translation / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / virus-mediated perturbation of host defense response / host cell nucleus / membrane Similarity search - Function | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Bruns, K. / Studtrucker, N. / Sharma, A. / Fossen, T. / Mitzner, D. / Eissmann, A. / Tessmer, U. / Roder, R. / Henklein, P. / Wray, V. / Schubert, U. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2007 Title: Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein. Authors: Bruns, K. / Studtrucker, N. / Sharma, A. / Fossen, T. / Mitzner, D. / Eissmann, A. / Tessmer, U. / Roder, R. / Henklein, P. / Wray, V. / Schubert, U. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2hn8.cif.gz | 279.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2hn8.ent.gz | 231.4 KB | Display | PDB format |
PDBx/mmJSON format | 2hn8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2hn8_validation.pdf.gz | 335.4 KB | Display | wwPDB validaton report |
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Full document | 2hn8_full_validation.pdf.gz | 457.7 KB | Display | |
Data in XML | 2hn8_validation.xml.gz | 26.1 KB | Display | |
Data in CIF | 2hn8_validation.cif.gz | 40.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hn/2hn8 ftp://data.pdbj.org/pub/pdb/validation_reports/hn/2hn8 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4848.931 Da / Num. of mol.: 1 / Fragment: C-terminal domain, amino acid residues 50-87 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence of the peptide is naturally expressed/found in cells infected with Homo sapiens (human) Influenza A virus strain A/Puerto Rico/8/34 (H1N1). References: UniProt: P0C0U1 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 2 mM PB(50-87), 300 K, pH 3, 50% H2O, 50% TFE-d2 / Solvent system: 50% H2O, 50% TFE-d2 |
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Sample conditions | Ionic strength: 0 / pH: 3 / Pressure: ambient / Temperature: 300 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |